Substrate for fluoroimmunoassay of biological fluids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436518, 436528, 436531, 436535, G01N 3354

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active

045406600

ABSTRACT:
A substrate for fluoroimmunoassay is disclosed comprising a swellable, rehydratable polymeric protein-binding material forming a three-dimensional matrix; first particulate, light scattering centers distributed in the polymeric matrix capable of scattering visible light; and second particulate, light-scattering centers distributed in the polymeric matrix capable of reflecting fluorescent excitation light and absorbing all other light. The substrate efficiently binds proteins and enhances transmitted fluorescent light and thus increases the sensitivity of assays involving fluorescent measurements. The polymeric protein binding material is preferable an acrylic copolymer. The first light scattering centers are preferably oxides of titanium and zinc, and the second light-scattering centers are preferably a phthalocyanin compound.

REFERENCES:
patent: 4340564 (1982-07-01), Harte et al.
patent: 4363634 (1982-12-01), Schall
patent: 4468371 (1984-08-01), Chen et al.
Journal of Applied Physics, vol. 52, (10), pp. 6197-6202, Popovic, Z. D.

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