Substrate for determining leucine aminopeptidase or gamma-glutam

Details Substrate for determining leucine aminopeptidase or gamma-glutam Substrate for determining leucine aminopeptidase or gamma-glutam

1988-09-20

1989-10-31

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, C12Q 136, C12Q 126

Patent

active

048777277

ABSTRACT:
Leucine aminopeptidase (LAP) activity or .gamma.-glutamyltranspeptidase (.gamma.-GTP) activity in a sample is determined by converting a substrate represented by the formula (I): ##STR1## wherein Z represents (CH.sub.3).sub.2 CHCH.sub.2 CH(NH.sub.2)--or (NH.sub.2)(COOH) CHCH.sub.2 CH.sub.2 --, R.sub.1 represents hydrogen, alkyl or substituted alkyl, R.sub.2 represents alkylene or hydroxyalkylene, R.sub.3 and R.sub.4 are the same or different and represents hydrogen, halogen, nitro, hydroxyl, sulfo, carboxyl, alkyl, and alkoxy, and salts thereof into a compound represented by the formula (II): ##STR2## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 have the same meanings as defined above. The compound (II) is reacted with a chromogen to form a pigment or converted into a diazonium salt which is reacted with a coupling agent to form an azo dye. Absorption of a resultant colored reaction solution is measured.

REFERENCES:
patent: 4588836 (1986-05-01), Matsumoto et al.
patent: 4675290 (1987-06-01), Matsumoto et al.

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