Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-07-28
1997-07-29
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, C12Q 168, C07H 2104
Patent
active
056521057
ABSTRACT:
A substrate selective for detection of mammalian 5-C-DNA methyltransferase in the presence of bacterial 5-C-DNA methyltransferase, said substrate comprising oligomeric DNA which contains at least one 5-methylcytosine residue, and at least one cytosine or 5-fluorocytosine residue, each of which are followed in linkage to a guanine residue. The invention also includes a method for measuring the presence of mammalian 5-C-DNA methyltransferase which comprises contacting a sample containing 5-C-DNA methyl transferase with the substrate and also includes a method for inhibiting mammalian 5-C-DNA methyltransferase which comprises contacting a sample containing 5-C-DNA methyl transferase with the substrate.
REFERENCES:
patent: 5503975 (1996-04-01), Smith et al.
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Klimasauskas, et al. "Hhal Methyltransferase Flips Its Target Base Out of the DNA Helix" Cell, vol. 76, pp. 357-369, 1994.
Christman, et al. "5-Methyl-2'-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation" Proc. Natl. Acad. Sci. vol. 92 pp. 1-5, 1995.
Abstract of Sheikhnejad, et al. "A novel Mechanisms for de novo Methylation of DNA is Suggested by Evidence that 5-methylcytosine [5mC] Residues in Specific Sites in ss-DNA direct methylation of Cytosine[C] Residues in Distant CpG sites" PAACR vol. 35, 1994.
Sheikhnejad, et al. "Elucidation of Sequence Recognition by Murine C5-DNA Methyltransferase Using Defined Oligonucleotide Substrates Containing 5-Methyl-Deoxycytidine and 5-Fluoro-Deoxycytidine at Specific Sites" Mol. & Cell. Diff. vol. 1, p. 383, 1993.
Christman Judith K.
Marasco, Jr. Canio J.
Sheikhnejad Gholamreza
Sufrin Janice R.
Dunn Michael L.
Health Research , Inc.
Robinson Douglas W.
Wai Thanda
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