Substrate conversion with an enzyme immobilized on an ultrafiltr

Details Substrate conversion with an enzyme immobilized on an ultrafiltr Substrate conversion with an enzyme immobilized on an ultrafiltr

1989-06-19

1992-07-14

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

426 50, 426 52, 435 41, 435 98, 435176, 435180, 435288, 435803, C12P 1920, C12N 1114, C12N 1108, C12M 140

Patent

active

051302373

ABSTRACT:
A process is disclosed for chemically converting a substrate into its reaction products and immediately thereafter physically separating the reaction products in a continuous operation. The process is carried out with a bioreactor having an ultrafiltration membrane containing an immobilized chemical agent which is preferably an enzyme. The bioreactor is prepared by securing an ultrafiltration membrane to an inside wall of a porous tubular support and chemically bonding an enzyme to an inner surface of the membrane. The enzyme is preferably bonded to the membrane by chelation and the membrane may be a polymeric membrane or a metal oxide membrane. To convert a substrate, a substrate-containing feed stream is preferably flowed tangentially along the inner surface of the membrane containing the immobilized enzyme. Sufficiently small reaction products filter through pores of the membrane and larger reaction products are retained by the membrane. In a preferred embodiment, contacting of fresh fruit juice with pectinase immobilized on the membrane results in pectinase treatment or the juice and immediate extraction and clarification of the juice. In another embodiment, the enzyme immobilized is glucoamylase and corn dextrins are converted to reducing sugars.

REFERENCES:
patent: 4001085 (1977-01-01), Keyes
patent: 4033822 (1977-07-01), Gregor
patent: 4266026 (1981-05-01), Breslau
patent: 4716044 (1987-12-01), Thomas et al.
patent: 4897465 (1990-01-01), Cordle et al.
patent: 4957890 (1990-09-01), Wieserman et al.
patent: 4962073 (1990-10-01), Martin et al.

Affiliated with

Also associated with

Rating

Substrate conversion with an enzyme immobilized on an ultrafiltr does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Substrate conversion with an enzyme immobilized on an ultrafiltr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substrate conversion with an enzyme immobilized on an ultrafiltr will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-334129