Compositions: coating or plastic – Coating or plastic compositions – Proteinaceous material containing
Patent
1984-03-07
1986-01-21
Bell, James J.
Compositions: coating or plastic
Coating or plastic compositions
Proteinaceous material containing
2601237, C08L 8900
Patent
active
045655804
ABSTRACT:
A substrate consisting essentially of regenerated collagen fibrils is provided which is in the form of a bead, or microsphere and comprises irregularly entangled regenerated collagen fibrils each having a diameter of 10-1000 m.mu. and an aqueous solution existing between the regenerated collagen fibrils, the content of the regenerated collagen fibrils being 20-0.01 wt. %. The substrate can be used for cell culture or for measuring adhesion activity of blood platelet.
According to one method of manufacturing the collagen beads, an acidic aqueous collagen solution is dispersed in a water-immiscible organic solvent in the form of numerous droplets to form an emulsion, and the droplets are then coagulated by addition of a water-miscible organic solvent and an alkali to the emulsion.
According to another method of manufacturing the collagen beads or microspheres, a neutral collagen solution is dispersed in a water-immiscible organic solvent in the form of numerous droplets to form an emulsion, and the droplets are then coagulated by raising the temperature of the emulsion to 30.degree. C.-40.degree. C. The collagen beads or microspheres prepared by the methods described above may be cross-linked by hexamethylenediisocyanate or glutaraldehyde.
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Develop., Growth and Differ., 23 (2), pp. 157-184 (1981), "In Vitro Orientation of Fibroblasts and Myoblasts on Aligned Collagen Film".
Miyata Teruo
Namiki Shin-ichi
Bell James J.
Koken Co. Ltd.
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