Substrate composition for solid phase urease immunoassay

Details Substrate composition for solid phase urease immunoassay Substrate composition for solid phase urease immunoassay

1992-03-16

1994-07-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435 791, 435188, 436904, B12Q 158, C12Q 100, C12N 996

Patent

active

053288319

ABSTRACT:
A substrate composition for use in a solid phase enzyme assay for urease or in a solid phase enzyme immunoassay which includes urease as a label. The substrate composition includes a compound converted by urease to ammonia and a pH dependent reducing agent which reduces a tetrazolium salt when the pH of the medium has been raised by the ammonia produced. The tetrazolium salt may optionally be included in the substrate composition. Reduction of the tetrazolium salt produces a colored insoluble formazan which precipitates on the solid phase as an indication of the presence of urease.

REFERENCES:
patent: 4892817 (1990-01-01), Pawlak
patent: 4946776 (1990-08-01), Ritterband

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