Substrate composition for alkaline phosphatase and method for as

Details Substrate composition for alkaline phosphatase and method for as Substrate composition for alkaline phosphatase and method for as

1988-11-18

1992-08-04

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 4, 435810, 435174, 435176, 435177, 4353171, 435975, 435 79, 435 794, 435 793, 435 792, 435 772, 435 21, 424 71, 424 88, 514368, C12G 100, C12N 1100, A61K 3912

Patent

active

051358476

ABSTRACT:
A composition contains levamisole and a substrate for alkaline phosphatase in a high pH buffer including 2-amino-2-methyl-1-propanol. The composition may be included in a kit of materials useful in performing an immunoassay in which intestinal alkaline phosphatase is the label. The invention includes a method for detection of a viral antigen by an immunoassay including intestinal alkaline phosphatase as the label and the composition of the invention to provide stabilized levamisole to inhibit nonintestinal alkaline phosphatase.

REFERENCES:
patent: 4322495 (1982-03-01), Kato
patent: 4693970 (1987-09-01), O'Connell
patent: 4847194 (1989-07-01), Quante
Ponder, B. A. et al., The J. Histoch. Cytochem. vol. 29, No. 8, pp. 981-984 (1981).
Voller, The Enzyme Linked Immunosorbent Assay (ELISA), MPEP Section 608.01(v).
USAN and the USP Dictionary of Drug Names.

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