Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-05-25
1992-08-18
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 793, 435 794, 435 795, 435 12, 435970, 436518, 436527, 436528, 436529, 436530, 436810, 436904, G01N 3353
Patent
active
051399342
ABSTRACT:
A method for enzyme immunoassay of a ligand includes urease as a label. A bound fraction of ligand and antiligand conjugated to urease is formed on a solid support. The urease component of the bound fraction is contacted with a substrate composition for urease which includes a compound converted to ammonia by the urease, a tetrazolium salt and a pH dependent reducing agent which reduces the tetrazolium salt when the pH of the assay medium has been raised by the ammonia. The tetrazolium salt is reduced to a colored insoluble formazan which precipitates as a detectable spot on the support. The invention includes the substrate composition and a kit of materials for performing the assay.
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Mapes James P.
Stewart Thomas N.
Vonk Glenn P.
Becton Dickinson and Company
Brown Richard E.
Fugit Donna R.
Kepplinger Esther L.
Spiegel Carol A.
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