Substrate and process for forming substrate for surface-enhanced

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic

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427287, 4273831, 427404, 427414, 422 57, 422 58, 436518, 436527, 436531, 436532, 436534, 436805, G01N 33553

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active

055277128

ABSTRACT:
A substrate for surface-enhanced analytical procedures, such substrates prepared for use in specific procedures to detect specific binding partner molecules, and a method for making them. The substrate is a body having a substrate surface on which there are randomly spaced apart metal islands. A continuous layer of coupling agent coats the islands and any intervening substrate surface. First binding partner molecules are bonded to the coupling agent. These are specifically attractive to specified second partner molecules for an analytical procedure for detecting the presence of, and perhaps the concentration of second partner molecules, or in some procedures, of a specified third binding partner molecule which becomes bonded to the second binding partner molecule. The thickness of the coupling agent layer is selected to improve the sensitivity of a given analytical procedure for detecting or measuring the specific binding partner molecule.

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D. A. Weitz et al, J. Electron Spectroscopy and Related Phenomena, 29 (1983), p. 363, A Comparison of Raman Scattering, Resonance Raman Scattering, and Flourescence from Molecules, absorbed on Silver Island Films.
T. M. Cotton et al, J. Physical Chemistry, 1986, 90-6071-6073 Distance Dependence of Surface-Enhanced Resonance Raman Enhancement in Langmuir-Blodgett Dye Multilayers.
Thomas E. Rohr et al, Analytical Biochemistry (1989), p. 182, Avidin-Coated Silver Films Demonstrated SERS with Antigen-Antibdy reactions. Antibody coated Directly on the Surface.
Dellerich, "Enzyme-Immunoassay: A Review", J. Clin. Chem. Clin. Biochem., vol. 22, 1984, pp. 895-904.

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