Substrate and method for producing the substrate

Coating processes – Applying superposed diverse coating or coating a coated base – Metallic compound-containing coating

Reexamination Certificate

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C435S007100

Reexamination Certificate

active

08071177

ABSTRACT:
A self assembling monolayer formed by the self assembling molecules is caused to bond onto the surface of a base material, and chelators are caused to bond onto the self assembling monolayer, to produce a substrate, in which the chelators are bound to the self assembling monolayer at a density within a range from 0.4
m2to 4
m2. The bonding of the chelators onto the self assembling monolayer being performed within an organic solvent.

REFERENCES:
patent: 6197515 (2001-03-01), Bamdad et al.
Valiokas et al., “Differential Protein Assembly on Micropatterned Surfaces with Tailored Molecular and Surface Multivalency,” ChemBioChem, 2006, vol. 7, Issue 9, pp. 1325-1329.
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Sigel et al., “A Self-Assembled Monolayer for the Binding and Study of Histidine-Tagged Proteins by Surface Plasmon Resonance,” Anal. Chem., 1996, vol. 68, No. 3, pp. 490-497.
“High Affinity Chelator Thiols for Switchable and Oriented Immobilization of Histidine-Tagged Proteins: A Generic Platform for Protein Chips Technologies”, A. Tinazli et al., Chem. Eur, J., vol. 11, pp. 5249-5259, 2005.
“Production of Histidine Tagged Protein Arrays and Label Free Interaction Observations”, M. Kyo and T. Natsume, Toyobo Life Science Magazine, vol. 77, pp. 15-16, 2004.
Applications, Properties and Synthesis of ω-Funchionalized n-Alkanethiols and Disulfides—the Building Blocks of Self-Assembled Monolayers, D. Witt et al., Curr. Org. Chem. vol. 8, pp. 1763-1797, 2004.
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“A Survey of Structure-Property Relationships of Surfaces that Resist the Adsorption of Protein”, E. Ostuni et al., Langmuir, vol. 17, pp. 5605-5620, 2001.
“Self-Assembled Monolayers That Resist the Adsorption of Proteins and the Adhesion of Bacterial and Mammalian Cells”, E Ostuni et al., Langmuir, vol. 17, pp. 6336-6343, 2001.
“Self-Assembled monolayers of Thiolates on Metals as a Form of Nanotechnology”, J.C. Love et al., Chemical Review, vol. 105, pp. 1103-1169.

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