Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-07-18
2006-07-18
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007100, C536S023100, C536S024300
Reexamination Certificate
active
07078172
ABSTRACT:
It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxide solution) of 1-[3-(dimethylamino) propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the spotted DNA with fluorescence labeled DNA.
REFERENCES:
patent: 5656462 (1997-08-01), Keller et al.
patent: 8-500722 (1996-01-01), None
patent: WO 99/66322 (1999-12-01), None
S. Löf{hacek over (a)}s and B. Johnson “A novel maix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilisation of ligands,” J Chem. Soc.. Chem. Commun, 1526-1528 (1990).
Silva Storri et al. “A Piezoelectric Biosensors for DNA Hybridisation Detection”Analytical letters31(11);1795-1808 (1998).
Mengsu Yang et al. “Covalent Immobilization of Oligonucleotides on Modified Glass/Silicon Surfaces for Solid-phase DNA Hybridization and Amplification”Chemistry Letter:257-258 (1998).
Japan Patent Information Organization “Chemically Modified Border Brim and Method of Manufacturing for the Same” (2001-139532) Dialog File Number 347 Accession No. 6911996.
Ohba Mitsuyoshi
Okamura Hiroshi
Takagi Ken-ichi
Tanga Michifumi
Yamakawa Kaoru
Browdy and Neimark PLLC
Toyo Kohan Co. Ltd.
Whisenant Ethan
LandOfFree
Substrate activation kit and method for detecting DNA and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Substrate activation kit and method for detecting DNA and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substrate activation kit and method for detecting DNA and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3584394