Substitutional variants of hydroxynitrile lyase with high...

Details Substitutional variants of hydroxynitrile lyase with high... Substitutional variants of hydroxynitrile lyase with high...

2005-10-14

2011-10-04

Fronda, Christian (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S183000, C435S320100, C536S023200

Reexamination Certificate

active

08030053

ABSTRACT:
An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

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