Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-10-27
1997-08-19
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 29, 435 6, 436 94, 436800, 536 2673, 536 111, 536 256, C12Q 104, C12Q 102, G01N 3300, C07H 1920
Patent
active
056587511
ABSTRACT:
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system having at least one substituent on the pyridinium or quinolinium ring that contains a heteroatom. The presence of the heteroatom-containing substituent results in higher sensitivity to oligonucleotides and larger nucleic acid polymers in a wide range of cells and gels, and for use in analysis of cell structure, membrane integrity or function.
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Haugland Richard P.
Jin Xiaokui
Jones Laurie J.
Millard Paul J.
Mozer Thomas J.
Helfenstein Allegra J.
Kight John
Leary Louise
Molecular Probes, Inc.
Skaugset Anton E.
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