Substituted unsymmetrical cyanine dyes with selected permeabilit

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 4, 435 29, 435 6, 436 94, 436800, 536 2673, 536 111, 536 256, C12Q 104, C12Q 102, G01N 3300, C07H 1920

Patent

active

056587511

ABSTRACT:
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system having at least one substituent on the pyridinium or quinolinium ring that contains a heteroatom. The presence of the heteroatom-containing substituent results in higher sensitivity to oligonucleotides and larger nucleic acid polymers in a wide range of cells and gels, and for use in analysis of cell structure, membrane integrity or function.

REFERENCES:
patent: 4665024 (1987-05-01), Mansour et al.
patent: 4883867 (1989-11-01), Lee et al.
patent: 4937198 (1990-06-01), Lee et al.
patent: 5321130 (1994-06-01), Yue et al.
patent: 5436134 (1995-07-01), Haugland et al.
Brooker, et al., J. Am. Chem. Soc. 64, 199 (1942).
Davidson, The Biochemistry of the Nucleic Acids (1976).
Wittung, et al., Nature 368, 561 (1994).
Diwu et al., Cytometry supp. 7, p. 77, Abstract 426B (1994).
Wawzonek, et al., J. Heterocyclic Chem. 25, 381 (1988).
Elderfield, Heterocyclic Compounds, vol. 4, pp. 1-331 (1952).
Marson, Tetrahedron., 48, 3659 (1992).
Saitoh, et al., Cell 76, 609 (1994).
Hongyo, et al., Nucleic Acids Research 21, 3637 (1993).
Noueiry, et al., Cell 76 925 (1994).
Perkins, et al., Science 264, 822 (1994).
Perkins et al., Science 264, 819 (1994).
Bensimon, et al., Science 265, 2096 (1994).
Goodwin, et al., Nucleic Acids Research 21, 803 (1993).
Castro, et al., Anal. Chem. 65, 849 (1993).
Kudinova, et al., Chemical Abstracts 93:241180j (1993).
Kudinova et al., Khim. Geterotsikl. Soedin. 7, 903 (1980).
Simbera, et al., Chemical Abstracts 89:112299y (1978).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Substituted unsymmetrical cyanine dyes with selected permeabilit does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Substituted unsymmetrical cyanine dyes with selected permeabilit, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substituted unsymmetrical cyanine dyes with selected permeabilit will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1103947

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.