Substituted phenylacetic acids and salts as TBP blocking agents

Details Substituted phenylacetic acids and salts as TBP blocking agents Substituted phenylacetic acids and salts as TBP blocking agents

1982-09-03

1984-08-28

Nucker, Christine M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

435 4, 435 7, 436537, 436546, 436804, 436808, 436826, G01N 3352, G01N 3354

Patent

active

044684690

ABSTRACT:
An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

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