Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-09-16
1991-09-10
Saunders, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 18, 435188, 435 792, 436512, 436547, 530388, 530391, G01N 33535, G01N 33563
Patent
active
050473247
ABSTRACT:
A method for obtaining a substantially pure preparation of an enzyme-antibody conjugate having an enzyme component covalently coupled to a predetermined number of an antibody component where the molecular weight of the enzyme component is substantially greater than the molecular weight of the antibody component. The method involves the electrophoretic separation of the desired enzyme-antibody conjugate from an enzyme-antibody conjugate reaction mixture comprising, as separately migratable species, at least a free enzyme component and a poplulation of enzyme-antibody conjugates comprising one or more of the enzyme component convalently coupled to one or more of the antibody component. The resulting conjugate preparations are substantially pure and are therefore particularly useful as labeled reagents in immunometric assays.
REFERENCES:
patent: 4551426 (1985-11-01), Freytag et al.
patent: 4657853 (1987-04-01), Freytag et al.
Chambrach, Methods of Protein Separation, vol. 2, 1976, pp. 105-106.
Chambrach, The Practice of Quantitative Gel Electrophoresis, VCH, 1985, pp. 157-158.
Kraehenbuhl et al, Journ. Cell Biol., 50, 432-445, 1971.
Avrameas, Immunochemistry, 6, 43-52, 1969.
Casu et al, Ital. Journ. Biochem. (Engl. Edn.), 18, 166-173, 1969.
Ram et al, Journ. Cell Biol. 17, 673-675, 1963.
Avrameas et al, Immunochemistry, 8, 1175-1179, 1971.
Davies et al, Proc. Natl. Acad. Sci. U.S.A. 66, 651-656, 1970.
Tijssen, Practice and Theory of Enzyme Immunoassays, Elsevier, Amsterdam, 1985, pp. 265-270.
Mashida et al, Journal of Immunoassay, 6, 111-123, 1985.
Imagawa et al, Journal of Biochemistry, 96, 1727-1735, 1984.
Kennedy et al, Clinica Chimica Acta, 70, 1-31, 1976.
Pillai et al, Febs Letters, 90, 51-53, 1978.
Collins Daniel W.
Klawitter Andrew L.
Miles Inc.
Saunders David A.
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