Substance with lipo-oligosaccharide structure capable of acting

Plant husbandry – Coated or impregnated seed – method or apparatus

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47 58, 536 172, 536 221, 5361231, 435 84, 4351723, 4352522, 435878, 514 54, A01C 106, A01H 304, C12P 1904, C12R 141

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active

055497187

ABSTRACT:
An essentially pure substance has a structure of a Nod factor or one of its analogues. The Nod factor is characterized by the fact that its biosynthesis is controlled by at least one nodulation gene (nodA,B,C) common to the Rhizobiaceae, in particular to the genera Rhizobium, Bradyrhizobium, Sinorhizobium and Azorhizobium. This substance consists of a lipo-oligosaccharide which is not a derivative of the exopolysaccharides and which has the general formula (I). In formula (I), the Nod factor of which it has the structure is a plant-specific symbiotic signal and is capable of enhancing the capacity of the bacteria of infect the host plant with which it is associated and/or of accelerating the formation of nodules on the host plant with which it is associated and/or of inducing the transcription of symbiotic genes of the leguminoseae. Applications to the treatment of plants and as an active therapeutic agent in humans and animals. G stands for a hexosamine variously substituted, for example by an acetyl group on the nitrogen, a sulphate group, an acetyl group and/or an ether group on oxygen R.sub.1, R.sub.2, R.sub.3, R.sub.5, R.sub.6, R.sub.7, which may be identical or different, stand for H, CH.sub.3 CO--, C.sub.x H.sub.y CO-- where X is a whole number between 0 and 17 and Y is a whole number between 1 and 35, or any other acyl group, for example a carbamyl groups, R.sub.4 stands for a saturated or mono-, di or tri-unsaturated aliphatic chain containing at least 12 carbon atoms and n is a whole number between 1 and 4.

REFERENCES:
Chemical Abstracts, vol. 102, No. 3, 21 Jan. 1985, (Columbus, Ohio, US), M. Abe et al.: "Stimulation of Clover root hair infection by lectin-binding oligosacharides from the capsular and extracellular polysaccharies of Rhizobium trifolii", p. 416, abstract 21445p, & J. Bacteriol. 1984, 160(2), 517-20.
Chemical Abstract, vol. 110 No. 25, 19 Jun. 89, (Columbus, Ohio, US), p. 689, abstract 232023c, & JP, A. 63255294 (Ihara Chemical Industry Co., Ltd.) 21 Oct. 1988.
Nature, vol. 344, 19 Apr. 90, P. Lerouge et al.: "Symbiotic host-specificity of Rhizobium meliloti is determined by a suphated and acylated glucosamine oligosaccharide signal", pp. 781-784.
Journal of Bacteriology, vol. 172, No. 2, Feb. 1990, pp. 901-911, Mary A. Honma, et al., "Rhizobium Meliloti nod Genes Mediate Host-Specific Activation of nodABC".
Journal of Bacteriology, vol. 168, No. 3, Dec. 1986, pp. 1075-1086, Frederic Debelle, et al., "Assignment of Symbiotic Developmental Phenotypes To Common And Specific Modulation (nod) Genetic Loci of Rhizobium Meliloti".
The EMBO Journal, vol. 2, No. 6, pp. 947-952, J. Allan Downie, et al., "Cloning Of The Symbiotic Region Of Rhizobium Leguminosarum: The Modulation Genes Are Between The Nitrogenase Genes And a nifA-Like Gene" (1983).
Kosslak et al. 1987. PNAS USA 84: 7428-7432.
Truchet et al. 1985. J. Bacteriol. 164:1200-1210.
Faucher et al. 1988, J. Bacteriol. 170:5489-5499.

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