Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Reexamination Certificate
2008-02-01
2010-11-23
Gakh, Yelena G. (Department: 1797)
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
C436S164000, C436S171000
Reexamination Certificate
active
07838302
ABSTRACT:
The present invention generally relates to sub-diffraction limit image resolution. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction limit resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA, a protein, a cell, a tissue, etc.
REFERENCES:
patent: 6008373 (1999-12-01), Waggoner et al.
patent: 2002/0064789 (2002-05-01), Weiss et al.
patent: 2006/0038993 (2006-02-01), Hell
patent: WO 2006/127692 (2006-11-01), None
International Search Report from PCT application PCT/US2007/017618 mailed Dec. 15, 2008.
Antonik, M. et al. “Separating Structural Heterogeneities from Stochastic Variations in Fluorescence Resonance Energy Transfer Distributions via Photon Distribution Analysis” J. Phys. Chem. B, vol. 100, No. 13, Mar. 15, 2006, pp. 6970-6978.
Bates, M., et al. “Short-Range Spectroscopic Rules Based on a Single-Molucule Optical Switch” Physical Review Letters, vol. 94, No. 108101, Mar. 18, 2005, pp. 108101-1-108101-4.
Friedman, L. et al. “Viewing Dynamic Assembly of Molucular Complexes by Multi-Wavelength Single-Molecule Fluorescence” Biophysical Journal, vol. 91, May 12, 2006, pp. 1023-1031.
Habuchi, S., et al. “Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa” PNAS, vol. 102, No. 27, Jul. 5, 2005, pp. 9511-9516.
Hofmann, M., et al. “Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins” Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington, DC, vol. 102, No. 49, Dec. 6, 2005, pp. 17565-17569.
Rust, M. J., et al., Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),Nature Methods, pp. 1-3 (2006).
Amato, I., “Squint Busters: Tool builders are pushing optical microscope vision to single-molecule sharpness,”Chemical&Engineering News, Sep. 4, 2006, pp. 49-52.
Betzig, E. “Proposed method for molecular optical imaging,”Optics Letters, vol. 20, No. 3, Feb. 1, 1995, pp. 237-239.
Betzig, E., et al., “Imaging Intracellular Fluorescent Proteins at Nanometer Resolution,”ScienceExpress, Aug. 10, 2006, pp. 1-9 (2006).
Betzig, E., et al., “Imaging Intracellular Fluorescent Proteins at Nanometer Resolution (Supporting Online Material),” http:www.sciencemag.org/cgi/content/full/1127344/DC1, pp. 1-30 (2006).
Wang, W., et al., “Label-free detection of small-molecule-protein interactions by using nanowire nanosensors,”Proc. Natl. Acad. Sci. USA, vol. 102, No. 9, pp. 3208-3212 (2005).
“Frontiers in live cell imaging/NIGMS and the Cell Migration Consortium (Movie),” National Institute of General Medical Sciences, National Institute of General Medical Sciences, Apr. 20, 2006, http://videocast.nih.gov/launch.asp?13187.
Bates, Mark et al. “Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes” Current Opinion in Chemical Biology, 2008, 12, pp. 505-514.
Hess, Samuel T., et al. “Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy” Biophysical Journal, vol. 91, Dec. 2006 4258-4272.
Huang, Bo, et al. “Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy” Science, Feb. 8, 2008, vol. 319, pp. 810-813.
Juette, Manuel F. et al. “Three-dimensional sub-100 nm resolution fluorescence micrscopy of thick samples” Nature Methods, vol. 5, No. 6, Jun. 2008, p. 527-529.
Kao, H. Pin, et al. “Tracking of Single Fluorescent Particles in Three Dimensions: Use of Cylindrical Optics to Encode Particle Position” Biophysical Journal, Vol. 67, Sep. 1994, p. 1291-1300.
Lacoste, Thilo D., et al. “Ultrahigh-resolution multicolor colocalizationof single fluorescent probes” PNAS, Aug. 15, 2000, vol. 97, No. 17, pp. 9461-9466.
Prabhat, Prashant, et al. “Simultaneous imaging of several focal planes in fluorescence microscopy for the study of cellular dynamics in 3D” Proc. of SPIE, vol. 6090 (2006).
Speidel, Michael, et al. “Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging” Optics Letters, Jan. 15, 2003, vol. 28, No. 2, pp. 69-71.
Toprak, Erdal, et al. “Three-Dimensional Particle Tracking via Bifocal Imaging” Nano Letters, 2007, vol. 7, No. 7, pp. 2043-2045.
Van Oijen, A.M., et al. “3-Dimensional super-resolution by spectrally selective imaging” Chemical Physics Letters 292 (1998) 183-187.
Hell, S.W. et al. “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” J Opt Lett (1994) 19:780.
Huang, B. et al. “Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution,” Nat Meth (2008) 5:1047.
Pavani, S. et al. “Three Dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” PNAS (2009) 106, 2995.
Schmidt, R. et al. “Mitochondrial Cristae Revealed with Focused Light,” Nano Lett (2009) 9:2508.
Schmidt, R. et al. “Spherical nanosized focal spot unravels the interior of cells,” Nat Meth (2008) 5:539.
Shtengel, G. et al. “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure,” PNAS (2009) 106, 3125.
Vaziri, A. et al. “Multilayer three-dimensional super resolution imaging of thick biological samples,” PNAS (2008) 105, 20221.
International Office Communication in International Application No. 07872605.6, dated Jun. 15, 2009.
Office Action for U.S. Appl. No. 11/605,842, mailed Apr. 29, 2009.
Final Office Action for U.S. Appl. No. 11/605,842, mailed Nov. 6, 2009.
Dailey et al., “Confocal Microscopy of Living Cells”Handbook of Biol. Confocal Micr.381-403 (2006).
Bates Wilfred M.
Huang Bo
Rust Michael J.
Zhuang Xiaowei
Gakh Yelena G.
President and Fellows of Harvard College
Weisz David
Wolf Greenfield & Sacks P.C.
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