Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Patent
1995-06-09
1998-12-08
Lovering, Richard D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
264 41, 264 43, 264 44, 4272133, 42721333, 42721335, 4284022, 42840224, 435177, 435178, 435182, C12P 100, C12N 1104, C12N 1106, B01J 1320
Patent
active
058467629
ABSTRACT:
A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.
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Lockheed Martin Energy Research Systems, Inc.
Lovering Richard D.
Smirman Preston H.
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