Structural gene for membrane-bound aldehyde dehydrogenase

Details Structural gene for membrane-bound aldehyde dehydrogenase Structural gene for membrane-bound aldehyde dehydrogenase

1992-12-07

1999-06-22

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435140, 4353201, 4352523, 43525233, 536 232, C12N 904, C12N 1553, C12P 754

Patent

active

059142570

ABSTRACT:
This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said structural gene having a molecular size of about 3.6 Kb and having a restriction enzyme cleavage map as shown in FIG. 1; to a plasmid containing said structural gene; to an acetic acid bacterium transformed by said plasmid; and to an acetic acid fermentation process using said transformant.

REFERENCES:
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Agric. Biol. Chem., "Purification and Characterization of Aldehyde Dehydrogenase of Acetobacter Aceti", M. Ameyama et al., vol. 45, No. 8, 1981, pp. 1889-1890.
Chemical Abstracts, "Cloning of the Membrane-Bound Aldehyde Dehydrogenase Gene of Acetobacter Polyoxogenes and Improvement of Acetic Acid Production by Use of the Cloned Gene", M. Fukaya et al., vol. 110, No. 15, Apr. 10th 1989, p. 207, 129805k and Appl. Environ. Microbiol, 1989, 55(1), 171-6.
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Okumura, et. al.--Biochemical Characteristics of Spontaneous Mutants of Acetobacter aceti Deficient in Ethanol Oxidation--Agric. Biol. Chem. 49(8):2485-2487 (1987).
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