Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Reexamination Certificate
2003-05-12
2008-05-06
Warden, Jill (Department: 1797)
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
C436S086000
Reexamination Certificate
active
07368290
ABSTRACT:
The present invention relates to novel methods of determining the sequence and structure of proteins. Specifically, the present invention allows for the analysis of intact proteins within a mass spectrometer. Therefore, preparatory separations need not be performed prior to introducing a protein sample into the mass spectrometer. Also disclosed herein are new instrumental developments for enhancing the signal from the desired modified proteins, methods for producing controlled protein fragments in the mass spectrometer, eliminating complex microseparations, and protein preparatory chemical steps necessary for cross-linking based protein structure determination.Additionally, the preferred method of the present invention involves the determination of protein structures utilizing a top-down analysis of protein structures to search for covalent modifications. In the preferred method, intact proteins are ionized and fragmented within the mass spectrometer.
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Kruppa Gary
Schoeniger Joseph S.
Young Malin M.
Hill David M.
Moss Keri A
Sandia National Laboratories
Ward & Olivo
Warden Jill
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