Streptomyces promoter and method of use thereof

Details Streptomyces promoter and method of use thereof Streptomyces promoter and method of use thereof

1990-01-18

1992-11-17

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351721, 4351723, 43525235, 4353201, 536 27, 935 41, 935 43, C12P 2100, C12N 1500, C12N 1576, C12N 120

Patent

active

051643057

ABSTRACT:
A DNA sequence is described, which is a promoter for Streptomyces. This promoter is stronger than the wild type and ultimately increases transcription initiation and protein expression. Typically, a nucleotide base, such as guanine, is inserted into the promoter sequence between positions -50 and -75 to increase transcription initiation or protein expression. In a preferred embodiment, guanine is inserted between positions -62 and -63 of the promoter regulated by thiostrepton (tipA).

REFERENCES:
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Bibb et al., 1985, Mol. Gen. Genet. 199:26-36.
Murakami et al., Mar. 1989, J. Bacteriology 171(3):1459-1466.

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