Streptococcus pyogenes DNase B leader peptide and methods...

Details Streptococcus pyogenes DNase B leader peptide and methods... Streptococcus pyogenes DNase B leader peptide and methods...

2007-07-24

2007-07-24

Duffy, Patricia A. (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S069700, C435S071100, C435S320100, C435S252300, C435S252330, C435S252350, C536S023100, C536S023400

Reexamination Certificate

active

10453032

ABSTRACT:
The gene forStreptococcus pyogenesDNase B has been cloned and vectors incorporating the cloned DNA have been used to transformEscherichia coli,allowing efficient and rapid production of the DNase inE. coliwithout the necessity of growing large quantities ofS. pyogenes.The enzyme can be produced with a leader peptide at its amino terminus. An improved method for the purification of naturally occurringS. pyogenesDNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection byS. pyogenes.

REFERENCES:
patent: 3793149 (1974-02-01), Blank et al.
patent: 3920625 (1975-11-01), Anderson et al.
patent: 4421650 (1983-12-01), Nagasawa
patent: 4481291 (1984-11-01), Gils
patent: 4503035 (1985-03-01), Pestka et al.
patent: 4658017 (1987-04-01), Dembinski et al.
patent: 4832849 (1989-05-01), Cardin
patent: 5055395 (1991-10-01), Toth
patent: 5082785 (1992-01-01), Manning et al.
patent: 0613947 (1994-09-01), None
Sambrook et al, Molecular Cloning, A laboratory Manual, second edition, vol. 3, Chapter 17, Cold Spring Harbor Laboratory Press, 1989.
D. Collet-Cassart et al., “Turbidimetric Latex Immunoassay of Placental Lactogen on Microtiter Plates,”Clin. Chem.35(1):141-143 (1989).
A.C. Dwivedy et al , “Possible role of streptococci in pathogeneses of ankylosing spondylitus,”Indian J. Med. Res.88:475-479 (1988).
M.A. Gerber et al., “Enzyme-Linked Immunosorbent . . . DNase B,”J. Lab. Clin. Med.95:258-265 (1980).
E.L.V. Harris et al, Protein Purification Methods: A Practical Approach, IRL Press, pp. 56-65 (1989).
S. Horinouchi et al., “A New Isolation . . .Achromobacter lyticus,” Agric. Biol. Chem.41:2487-2489 (1977).
M. Iwasaki et al., “Cloning, Characterization and . . . Mitogenic Factor,”FEBS Lett.331:187-192 (1993).
G.C. Klein & W.L. Jones, “Comparison of the . . . Tests,”Appl. Microbiol.21:257-259 (1971).
R. Lathe, “Synthetic Oligonucleotide Probes Deduced from Amino Acid Sequence Data: Theoretical and Practical Considerations,”Journal of Molecular Biology, vol. 183, No. 1, pp. 1-111 (May 5, 1985).
J.E. Mott et al., “Maximizing Gene Expression . . . Factor D,”Proc. Natl. Acad. Sci. USA82:88-92 (1985).
J. Sambrook et al, “Molecular Cloning, A Laboratory Manual” (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1989) chs. 11-12 and 17.
E.D. Sevier et al., “Monoclonal Antibodies in Clinical Immunology,”Clin. Chem.27:1797-1805 (1981).
G. Sofer, “Chromatographic Removal of Pyrogens,”Bio/Technology: 1035-1038 (1984).
P. Tavernier et al, “Effects of Divalent Cations on Activity and Specificity of Streptococcal Nucleases B and D,”Biochemistry, vol. 9, No. 14, pp. 2846-2852 (1970).
T. Waldstrom et al, “Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis,”Proceedings of the International Symposium on Electrofocusing and Isotachophoresis, pp. 443-453 (1976 1977).
J.T. Whicher et al., Nephelometric Methods in “Practical Immunoassay, The State of the Art,” Marcel Dekker Inc. NY pp. 117-177 (1984).
J.M. Wozney, “Using Purified Protein to Clone Its Gene,”Methods Enzymol.182:738-749 (1990).
W.G. Yasminch, “Streptococcal Nucleases, V. Specificities of Deoxyribonuclease Action of the A, B, C. and D Enzymes,”Biochemistry, vol. 7, No. 1, pp. 105-113 (Jan. 1968).
R.A. Young & R.W. Davis, Efficient Isolation . . . Probes,Proc. Natl. Acad. Sci. USA80:1194-1198 (1983).
T. Yutsudo et al., “A New Type of Mitogenic Factor ProducedStreptococcus pyogenes,” FEBS Lett.308:30-34 (1992).

Affiliated with

Also associated with

Rating

Streptococcus pyogenes DNase B leader peptide and methods... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Streptococcus pyogenes DNase B leader peptide and methods..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Streptococcus pyogenes DNase B leader peptide and methods... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3776855