Streptavidin muteins

Details Streptavidin muteins Streptavidin muteins

1997-10-09

2000-08-15

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 71, 4353201, 435325, 4352523, 536 231, 530300, 530320, 530330, 530350, C12P 2106, C07H 1700, C07K 1400

Patent

active

061034932

ABSTRACT:
The invention concerns a polypeptide selected from muteins of streptavidin which is characterized in that it (a) contains at least one mutation in the region of the amino acid positions 44 to 53 with reference to wild type-(wt)-streptavidin and (b) has a higher binding affinity than wt-streptavidin for peptide ligands comprising the amino acid sequence Trp-X-His-Pro-Gln-Phe-Y-Z in which X represents an arbitrary amino acid and Y and Z either both denote Gly or Y denotes Glu and Z denotes Arg or Lys. In addition nucleic acids coding for the polypeptide, a vector containing this nucleic acid, a cell transfected with the vector as well as the use of a polypeptide in a method for the isolation, purification or determination of proteins are disclosed. Yet a further subject matter is a reagent kit containing the polypeptide.

REFERENCES:
Chilkoti et al. 1995 Proc. Natl. Acad. Sci. 92: 1754-1758.
Reznik et al. 1996 Nature Biotechnology 14: 1007-1011.
Reznik et al. Nov. 10 1998 Proc. Natl'l. Acad. Sci. 95: 13525-13530.
Sano et al. Jun. 10, 1997, Proc. Natl. Acad. Sci. 94:6153-6158.
Voss et al. Aug., 1997. Protein Eng. 10(8): 975-982.

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