Strategy for the production of RNA from immobilized templates

Details Strategy for the production of RNA from immobilized templates Strategy for the production of RNA from immobilized templates

1995-05-18

1997-12-23

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 915, C12P 1934

Patent

active

057006679

ABSTRACT:
The instant invention provides a method for producing RNA enzymatically in a semi-batch or continuous-flow bioreactor using a DNA template immobilized to a solid support through a linkage having a single-stranded overhang extending from a noncoding strand. The coding strand remains dissociable from the immobilized noncoding strand. The immobilized RNA template is reused in at least sixteen rounds of transcription reaction. The coding strand can be replaced with a fresh DNA strand encoding the same or a different RNA.

REFERENCES:
A.S. Spirin, in "Proceedings of Frontiers in Bioprocessing II," Boulder, Co, Jun. 17-21, Conference Proceedings Series (P. Todd, S.K. Sikdar and M. Bier, eds.), Amer. Chem. Soc., Washington, D.C. (1992).
Milligan et al., Nucleic Acids Res., vol. 15, No. 21, pp. 8783-8798, 1987.
Stratagene 1992 Product Catalog, p. 256, 1992.
Yolov et al. (1991), Bioorganiche skaya Xhimia 17(6): 789-794.
Fujita et al. (1993), Biotechniques 14(4): 608-617.
Arias et al. (1989), J. Biol. Chem. 264(6): 3223-3229.
Kigawa et al. (1991), J. Biochem. 110: 166-168.
Marble et al. (1993), Abstracts of Papers, 205th ACS National Meeting, Abstract BIOT 88.

Affiliated with

Also associated with

Rating

Strategy for the production of RNA from immobilized templates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Strategy for the production of RNA from immobilized templates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Strategy for the production of RNA from immobilized templates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1801921