Strand displacement amplification of RNA targets

Details Strand displacement amplification of RNA targets Strand displacement amplification of RNA targets

1997-05-08

1999-06-29

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 435 6, 536 2433, C12P 1934, C07H 2100

Patent

active

059167793

ABSTRACT:
Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.

REFERENCES:
patent: 5270184 (1993-12-01), Walker et al.
patent: 5322770 (1994-06-01), Gelfand
patent: 5455166 (1995-10-01), Walker
patent: 5523204 (1996-06-01), Singer et al.
patent: 5631147 (1997-05-01), Lohman et al.
J. W. Larrick "Message Amplification Phenotyping (MAPPing)--principles, practice and potential" Trends Biotech 10:146-152 (1992).
Dickson et al. Abstracts of the General Meeting of the American Society for Microbiology 96. 1996. 28.

Affiliated with

Also associated with

Rating

Strand displacement amplification of RNA targets does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Strand displacement amplification of RNA targets, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Strand displacement amplification of RNA targets will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1375027