Strand displacement amplification

Details Strand displacement amplification Strand displacement amplification

1992-01-09

1995-10-03

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 5, 435 6, C12P 1934

Patent

active

054551668

ABSTRACT:
This invention relates a nucleic acid target amplification and detection method which operates at a single temperature and makes use of a polymerase in conjunction with an endonuclease that will nick the polymerized strand such that the polymerase will displace the strand without digestion while generating a newly polymerized strand.

REFERENCES:
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patent: 4683202 (1987-07-01), Mullis
patent: 5270184 (1993-12-01), Walker et al.
Molecular Cloning: A Lab. Manual, Maniatis et al., Eds, Cold Spring Harbor Lab, Cold Spring Harbor, 1982, p. 142.
Walker et al. (Jan. 1992) Proc. Natl. Acad. Sci. vol. 89, pp. 392-396.
Taylor et al. (B) (1985) Nucl. Acids Res. vol. 13 (24) pp. 8765-8784.
Taylor et al. (A) (1985) Nucl. Acids Res., vol. 13 (24) 8749-8764.
Bethesda Research Lab. Catalog & Reference Guide, 1989, pp. 8, 17, 22.
EP 395,398 A2.
Kay L. Nakamaye et al., Nucleic Acids Research (1986).
Jon R. Sayers et al., Nucleic Acids Research (1988) 16:791.
J. R. Sayers et al., Genet. Eng. (1988) 10:109-122.

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