Strand displacement amplification

Details Strand displacement amplification Strand displacement amplification

1995-01-18

1998-01-27

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12P 1934

Patent

active

057121249

ABSTRACT:
This invention relates a nucleic acid target amplification and detection method which operates at a single temperature and makes use of a polymerase in conjunction with an endonuclease that will nick the polymerized strand such that the polymerase will displace the strand without digestion while generating a newly polymerized strand.

REFERENCES:
patent: 5270184 (1993-12-01), Walker
Taylor et al. Nucleic Acids Res. 13(24):8749-8764, 1985.

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