Strain of Trichoderma harzianum useful as nematode...

Details Strain of Trichoderma harzianum useful as nematode... Strain of Trichoderma harzianum useful as nematode...

2001-01-05

2002-11-05

Naff, David M. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

C435S242000, C435S945000, C424S093500, C424S405000

Reexamination Certificate

active

06475772

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to a novel strain of
Trichoderma harzianum
useful as nematode inhibitor, fungicide and plant growth promoter and a process for the isolation thereof. This novel strain of
Trichoderma harzianum
has been deposited at Central Institute of Medicinal and Aromatic Plants (CIMAP) Type Culture Collection bearing accession No. C-Th-U.
BACKGROUND OF THE INVENTION
From the time seeds/planting propagules are sown/planted in the fields and till their harvesting, plants are exposed to a variety of microorganisms in its environment. These may be beneficial or deleterious to the plants depending on the specific microbe. Plant beneficial microbes preventing the growth or action of plant deleterious microbes are called bio-pesticides and this form of disease prevention is called bio-control. As a subject, bio-control has become more popular during the past few years mainly because of the introduction of more stringent regulations for the use of chemical pesticides. However, it still lacks farmers acceptability; the main reason being that it does not offer any additional benefits over the conventional chemical control.
Trichoderma harzianum
has been used as a bio-control agent to protect the plants against root, seed and foliar diseases.
PRIOR ART RELATING TO THE INVENTION
Some efforts to make this system of plant disease management more attractive have been made and some strains of
Trichoderma harzianum
have been developed/identified providing extra benefits of
i) plant growth promotion (Chang et al., 1986
, Plant Disease
, 70,145-148)
ii) resistance to pesticides (Papavizas et al., 1982
, Phytopathology
, 72,126-132) so that the strain can be utilized along with the pesticides in Integrated Pest Management
iii) effectiveness against phytonematodes (Windham et. al, 1989
, Phytopathology
, 73, 493-495),
iv) induction/enhancement of rooting of stem cuttings in the nursery (Mackenzie et. al, 1995
, HortScience
, 30, 496-498).
Though, the strains produced in these above prior arts possess one of the said advantages, a strain/isolate having more than one property (as mentioned above) would be definitely useful for the farmers/growers. Adopting such system of management of plant diseases as such strain, apart from managing plant pathogenic fungi and phytonematodes, would also contribute to improve plant growth.
Experiments have been carried out and a new strain of
Trichoderma harzianum
has been developed with antagonistic and bio-control potential against soil-borne plant pathogenic fungi and phytonematodes and ability to improve/enhance plant growth.
OBJECTS OF THE INVENTION
The main object of the present invention is to develop a novel strain of
Trichoderma harzianum
which has been deposited at CIMAP Type Culture Collection having accession No. C-TH-U and has been deposited at the American Type Culture Collection (ATCC), Manassas, Va. 20110-2209, USA, on Sep. 11, 2001, having accession No. PTA-3701, with a broader range of antagonistic activities like effectiveness against pathogenic fungi as well as nematodes etc.
Another object of the present invention is to develop novel strain of
Trichoderma harzianum
with additional advantages of plant growth promotion.
Yet another object of the present invention is to isolate a novel strain of
Trichoderma harzianum
from CIMAP field station at Bangalore, India.
SUMMARY OF THE INVENTION
To meet the above objects, the present invention provides a novel strain of
Trichoderma harzianum
and a process for the isolation of the same.


REFERENCES:
Chang et al., “Increased Growth of Plants in the Presence of the Biological Control AgentTrichoderma harzianum,” Plant Disease, pp. 145-147 (Feb. 1986).
Papavizas et al., “Evaluation of New Biotypes ofTrichoderma harzianumfor Tolerance to Benomyl and Enhanced Biocontrol Capabilities,”Phytopathology, vol. 72, No. 1, pp. 126-131 (1982).
Windham et al., “Effects ofTrichodermaspp. on Maize Growth andMeloidogyne arenariaReproduction,”Phytopathology, pp. 493-495 (Jun. 1989).
MacKenzie et al., “Enhanced Root and Shoot Growth of Chrysanthemum Cuttings Propagated with the FungusTrichoderma harzianum,” HortScience, vol. 30, No. 3, pp. 496-498 (Jun. 1995).

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