Strain of the western equine encephalitis virus

Details Strain of the western equine encephalitis virus Strain of the western equine encephalitis virus

2001-12-21

2004-10-05

Salimi, Ali R. (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

C435S091100

Reexamination Certificate

active

06800289

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to the cloning, sequencing and expression of the structural genes of western equine encephalitis (WEE) virus strain 71V-1658 and the development and use of the DNA-based vaccine against WEE.
BACKGROUND OF THE INVENTION
LIST OF PRIOR ART LITERATURES
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The alphaviruses are a group of about 27 enveloped viruses with a positive sense, nonsegmented single-stranded RNA genome (Calisher et al., 1980; Strauss and Strauss, 1988). The alphavirus disclosed in this invention, western equine encephalitis virus (WEE), is a member of the WEE antigenic complex and is serologically related to the Sindbis (SIN), Highlands J (HJ), Fort Morgan, Buggy Creek, and Aura viruses (Calisher & Karabatsos, 1988; Calisher et al., 1988). WEE is endemic in western North America and strains/varieties have been isolated from Argentina (AG80-646), Brazil (BeAr 102091) and the former Soviet Union (Y62-33) (Johnson and Peters, 1996; Weaver et al., 1997). In nature, WEE is transmitted from its amplifying hosts or reservoir in wild birds, to man and horses, by mosquitoes (
Culex tarsalis
being the principal vector). While the endemic cycle has resulted in only a limited number of human infections in recent years, in the past, major epidemics of WEE have been recorded. The most extensive epidemic, including 3,336 recognized human cases and 300,000 cases of encephalitis in horses and mules, occurred in the western United States and Canada in 1941 (Reisen & Monath, 1988; Johnson and Peters, 1996).
All alphaviruses share a number of structural, sequence, and functional similarities, including a genome with two polyprotein gene clusters (reviewed in Strauss & Strauss, 1994; Schlesinger & Schlesinger 1996). The genomic organization of these viruses is conserved (see FIG.
1
), with the nonstructural proteins translated directly from the 5′ two-thirds of the genomic RNA. A subgenomic positive-stranded RNA (the 26S RNA), is identical to the 3′ one-third of the genomic RNA and serves as the translational template for the structural proteins (capsid, E3, E2,6K and E1).
The nonstructural proteins (nsP1, nsP2, nsP3 and nsP4) are also synthesized as a polyprotein

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