Strain of the western equine encephalitis virus

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

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C435S091100

Reexamination Certificate

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06800289

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to the cloning, sequencing and expression of the structural genes of western equine encephalitis (WEE) virus strain 71V-1658 and the development and use of the DNA-based vaccine against WEE.
BACKGROUND OF THE INVENTION
LIST OF PRIOR ART LITERATURES
Ausubel, F. M., et al, editors. (1995).
Current Protocols in Molecular Biology
, New York: John Wiley & Sons.
Bell, J. R., Bond, M. W., Nukapiller, M. B., Strauss, E. G., Strauss, J. H., Yamamoto, K, & Simizu, B. (1983). Structural proteins of western equine encephalitis virus: amino acid compositions and N-terminal sequences.
Journal of Virology
45, 708-714.
Bird, B. R. & Forrester, F. T. (1981).
Basic Laboratory Techniques In Cell Culture
. Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control.
Calisher, C. H. & Karabatsos, N. (1988). Arbovirus serogroups: definition and geographic distribution. In
The Arboviruses: Epidemiology and Ecology
, Vol. I,.pp. 19-57. Edited by T. P Monath. CRC Press: Boca Raton, Fla.
Calisher, C. H., Shope, R. E, Brandt, W., Casals, J., Karabatsos, N., Murphy, F. A., Tesh, R. B., & Wiebe, M. E. (1980). Proposed antigenic classification of registered arbovirusess.
Intervirology
14, 229-232.
Calisher, C. H., Karabatsos, N., Lazuick, J. S. Monath, T. P., & Wolff, K. L. (1988). Reevaluation of the western equine encephalitis antigenic complex of alphaviruses (family Togaviridae) as determined by neutralization tests.
American Journal of Tropical Medicine and Hygiene
38, 447-452.
Cilnis, M. J., Kang, W. & Weaver, S. C. (1996). Genetic conservation of Highlands J viruses.
Virology
218, 343-351.
Frohman, M. A., Dush, M. K & Martin, G. R. (1988). Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer.
Proceedings of the National Academy of Science USA
85, 8998-9002.
Hahn, C. S., Lustig, S., Strauss, E. G. & Strauss, J. H. (1988). Western Equine Encephalitis virus is a recombinant virus.
Proceedings of the National Academy of Science USA
85, 5997-6001.
Johnson, R. E. & Peters, C. J. (1996). Alphaviruses. In
Fields Virology,
3rd edn, pp. 843-898. Edited by B. N. Fields, et al., New York: Raven Press.
Kuhn, R., Hong, Z. & Strauss, J. H. (1990). Mutagenesis of the 3′ nontranslated region of Sindbis virus RNA.
Journal of Virology
64, 1465-1476.
Kuhn, R. J., Niesters, H. G. M., Hong, Z. & Strauss, J. H. (1991). Infectious RNA transcripts from Ross River virus cDNA clones and the construction and characterization of defined chimeras with Sindbis.
Virology
182, 430-441.
Krieg, A. M., Yi, A.-K., Schorr, J. and Davis, H. L. (1998). The role of CpG dinucleotides in DNA vaccines.
Trends Microbiol.
6, 23-27.
McCluskie, M. J., Davies, H. L. (1999). Novel strategies using DNA for the induction of mucosal immunity.
Critic. Rev. in Immunol.
19, 303-329.
Ou, J.-H., Trent, D. W. & Strauss, J. H. (1982). The 3′ non-coding regions of alphavirus RNAs contain repeating sequences. Journal of Molecular Biology 156, 719-730.
Ou, J-H., Strauss, E. G. & Strauss, J. H. (1983). The 5′ terminal sequences of the genomic RNAs of several alphaviruses. Journal of Molecular Biology 168, 1-15.
Pardoll, D R, Beckering, A M. (1997). Exposing the immunology of naked DNA vaccines.
Immunity
3;165-169.
Pfeffer, M., Proebster, B., Kinney, R. M. & Kaaden, O-R. (1997). Genus-specific detection of alphaviruses by a semi-nested reverse transcription reaction. American Journal of tropical Medicine and Hygiene 57, 709-718.
Pfeffer, M., Kinney, R. M. & Kaaden, O-R. (1998). The alphavirus 3′-nontranslated region: Size heterogeneity and arrangement of repeated sequence elements. Virology 240, 100-108.
Prayaga, S. K., Fuller, D. H., Haynes, J. R. & Murphey-Corb, M. (1995). Particle-mediated nucleic acid immunization.
Vaccines
95, 105-109.
Reisen, W. K & Monath, T. P. (1988). Western equine encephalomyelitis, pp. 89-137
. In The Arboviruses: Epidemiology and Ecology, Vol. V
. Edited by T. P. Monath. CRC Press: Boca Raton, Fla.
Robinson, H. L., Feltquate, D. M., Morin, M. J., Haynes, J. R., Webster, R. G. (1995). DNA vaccines: A new approach to immunization.
Vaccine
95:69-75.
Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989).
Molecular Cloning, a Laboratory Manual,
2
nd edn
. Cold Spring Harbor: Cold Spring Harbor Laboratory.
Schlesinger, S. & Schlesinger, M. J. (1996). Togaviridae: The viruses and their replication, In
Fields Virology,
3rd edn, pp. 825-841. Edited by B. N. Fields, et al. New York: Raven Press.
Strauss, J. H., & Strauss, E. G. (1988). Evolution of RNA viruses.
Annual Review of Microbiology
42, 657-683.
Strauss, J. H., & Strauss, E. G. (1994). The alphaviruses: gene expression, replication, and evolution.
Microbiological Review
58, 491-562.
Strauss, E. G., Rice, C. M. & Strauss, J. H. (1983). Sequence coding for the alphavirus nonstructural proteins is interrupted by an opal termination codon.
Proceedings of the National Academy of Science USA
80, 5271-5275.
Strauss, E. G., Rice, C. M. & Strauss, J. H. (1984). Complete nucleotide sequence of the genomic RNA of Sindbis virus.
Virology
133, 92-110.
Trent, D. W., & Grant, J. A. (1980). A comparison of new world alphaviruses in the western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques.
Journal or General Virology
47:261-282.
Weaver, S. C., Hagenbaugh, A., Bellew, L. A., Netesov, S. V., Volchokov, V. I., Chang, G.-J J., Clarke, D. K., Gousset, L., Scott, T. W., Trent, D. W. & Holland, J. J. (1993). A comparison of the nucleotide seqeunces of eastern and western equine encephalomyelitis viruses with those of other alphaviruses and related RNA viruses.
Virology
197, 375-390.
Weaver, S. C., Kang, W, Shirako, Y., Rumenapf, T., Strauss, E. G. & Strauss, J. H. (1997) Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses.
Journal of Virology
71,613-623.
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Aesasi, G., Jani, A., Felgner, P. L. (1990). Direct gene transfer into mouse muscle in vivo.
Science
247,1465-1468.
The alphaviruses are a group of about 27 enveloped viruses with a positive sense, nonsegmented single-stranded RNA genome (Calisher et al., 1980; Strauss and Strauss, 1988). The alphavirus disclosed in this invention, western equine encephalitis virus (WEE), is a member of the WEE antigenic complex and is serologically related to the Sindbis (SIN), Highlands J (HJ), Fort Morgan, Buggy Creek, and Aura viruses (Calisher & Karabatsos, 1988; Calisher et al., 1988). WEE is endemic in western North America and strains/varieties have been isolated from Argentina (AG80-646), Brazil (BeAr 102091) and the former Soviet Union (Y62-33) (Johnson and Peters, 1996; Weaver et al., 1997). In nature, WEE is transmitted from its amplifying hosts or reservoir in wild birds, to man and horses, by mosquitoes (
Culex tarsalis
being the principal vector). While the endemic cycle has resulted in only a limited number of human infections in recent years, in the past, major epidemics of WEE have been recorded. The most extensive epidemic, including 3,336 recognized human cases and 300,000 cases of encephalitis in horses and mules, occurred in the western United States and Canada in 1941 (Reisen & Monath, 1988; Johnson and Peters, 1996).
All alphaviruses share a number of structural, sequence, and functional similarities, including a genome with two polyprotein gene clusters (reviewed in Strauss & Strauss, 1994; Schlesinger & Schlesinger 1996). The genomic organization of these viruses is conserved (see FIG.
1
), with the nonstructural proteins translated directly from the 5′ two-thirds of the genomic RNA. A subgenomic positive-stranded RNA (the 26S RNA), is identical to the 3′ one-third of the genomic RNA and serves as the translational template for the structural proteins (capsid, E3, E2,6K and E1).
The nonstructural proteins (nsP1, nsP2, nsP3 and nsP4) are also synthesized as a polyprotein

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