Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces

Details Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces

1997-07-25

1999-03-02

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4352521, C12P 1904, C12N 112

Patent

active

058769820

DESCRIPTION:

BRIEF SUMMARY
This application is a 371 of PCT/FR96/00127 filed Jan. 25, 1996.


BACKGROUND OF THE INVENTION

1. Field of the Invention
The invention concerns a new strain of Klebsiella pneumoniae, subsp. pneumoniae, and a process for the production of a polysaccharide containing L-fucose using this strain.
The new strain of Klebsiella pneumoniae subsp. pneumoniae (hereinafter termed BEC 1000) of the invention was deposited in the Collection Nationale de Cultures de Microorganismes, 25, rue du Docteur Roux, 75724 PARIS CEDEX 15, France, on 16 Dec. 1994, in accordance with the provisions of the Treaty of Budapest. It was given the registration number I-1507.
2. Technological Background
The use of bacterial strains of the genus Klebsiella to produce polysaccharides containing L-fucose is known in itself.
In Carbohydrate Research, 77 (1979), pages 183-190, J. P. JOSELEAU and M. -F. MARAIS describe a capsular polysaccharide containing D-galactose, L-fucose and D-galacturonic acid in a ratio of 1:1:1 originating from a strain of Klebsiella K-63.
In U.S. Pat. No. 4,298,691, G. T. VEEDER and K. S. KANG describe a process for the production of a S-156 heteropolysaccharide by culture of a strain of Klebsiella pneumoniae, close to the above strain K-63, deposited in the American Type Culture Collection, on 12.sup.th May 1988, as N.degree. ATCC 31646, in an aqueous nutrient medium by aerobic fermentation of a source of assimilatable glucides, such as hydrolysed starch. That heteropolysaccharide contains D-galacturonic acid, D-galactose and L-fucose in an approximate molar ratio of 23:21:26.


SUMMARY OF THE INVENTION

Following intensive research, the present inventors have discovered a new strain of K. pneumoniae subsp. pneumoniae, called BEC 1000, which has been shown to be capable of producing industrially useful quantities of a polysaccharide containing L-fucose which has properties which are of interest to the cosmetics industry, such as feel, moisturising ability, emulsion stabilisation capacity and perfume persistency power.


DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS OF THE INVENTION

Strain BEC 1000 has been isolated from the sludge from a sewerage plant. A sample of 1 g of sludge was used to inoculate 10 ml of culture medium placed in a 100 ml conical flask. The culture medium contained, per liter, 0.02 g of yeast extract, 10 g of glucose, 9 g of K.sub.2 HPO.sub.4, 3 g of KH.sub.2 PO.sub.4, 1 g of (NH.sub.4).sub.2 SO.sub.4, and 0.2 g of MgSO.sub.4,7H.sub.2 O, had a pH of 7.2 and had been autoclaved at 110.degree. C. for 30 minutes before use. The culture period was 6 days at 30.degree. C., with stirring at 120 rpm. A series of increasing dilutions of the culture medium was prepared and each was plated onto Petri dishes containing culture medium to which 2% by weight of agar had been added. After two days, colonies with a pronounced mucoid appearance were isolated.
The strain of the invention was selected after re-culturing the strongly mucoid colonies in culture medium because it provided a particularly positive response to the following determinations:
determination of 6-deoxyhexoses (methylpentoses) using the spectrophotometric method of Z. DISCHE and L. B. SHETTLES: "A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination", J. Biol. Chem. 175, 595-603 (1948);
determination of L-fucose by thin layer chromatography (TLC);
determination of L-fucose by high-pressure liquid chromatography (HPLC);
The BEC 1000 strain of the invention has the following characteristics: broth aeration and stirring)


______________________________________ NaCl 0% 3 3 3 NaCl 2% 3 3 3 NaCl 4% 3 3 3 NaCl 6% 3 3 3 NaCl 8% 2 2 2 NaCl 10% 0 1 1 ______________________________________


______________________________________ 5.degree. 0 0 0 10.degree. 0 1 2 15.degree. 1 2 3 30.degree. 3 3 3 41.degree. 3 3 3 45.degree. 3 3 3 Prototrophic culture 3 3 3 ______________________________________ 7H.sub.2 O: 200 mg;


______________________________________ CATALASE 3 OXIDASE REACTION

REFERENCES:
patent: 4298691 (1981-11-01), Veeder et al.
patent: 5330903 (1994-07-01), Kerr et al.
patent: 5336619 (1994-08-01), Matsuyama et al.
patent: 5354671 (1994-10-01), Pollock
Bergey's Manual of Systematic Bacteriology 1984, vol. 1, pp. 463-465.
R. T. Engeish "Ornithie-sorbitol agar as an aid to the identification of Kl. penumoniae in sputum" Medical Laboratory Science (1980) 37, pp. 365-366.
Structure of the Capsular Polysaccharide of Klebsiella K-Type 63, Jean-Paul Joseleau and Marie-France Marais; vol. 77, pp. 183-190 (1979), Carbohydrate Res.

Affiliated with

Also associated with

Rating

Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Strain of Klebsiella pneumoniae, subsp. pneumoniae, and a proces will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-421709