Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1998-06-01
2000-10-17
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
600 33, A01N 102, A61B 1743
Patent
active
061329525
ABSTRACT:
A procedure for cryopreservation and efficient post-thaw recovery of a single or few cells, preferably human spermatozoa, their precursor cells or blastomeres, which is achieved by injecting cells into cell-free mammalian zonae pellucidae. The cells may also be of any other type where there are provided a relatively small number of valuable cells which must be retrieved. The zona pellucida may be cryopreserved with the cells contained therein. The method involves a combination of physical micromanipulation procedures and cryoprotectant mediated cryopropreservation. The zonae may be tracked by positioning them into straws between two small air bubbles prior to preservation. There is a high recovery of zonae and sperm within the zonae, and a high fertilization rate of the sperm using ICSI after recovery.
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Cohen Jacques
Willadsen Steen
Saint Barnabas Medical Center
Saucier Sandra E.
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