Chemistry: analytical and immunological testing – Rate of reaction determination
Patent
1981-08-26
1983-08-16
Wyse, Thomas G.
Chemistry: analytical and immunological testing
Rate of reaction determination
436 53, G01N 3114
Patent
active
043992250
ABSTRACT:
A continuous method of quantitatively determining slowly reacting compounds with the use of a single measuring cell, the sample being injected into a continuously flowing carrier solution which is already provided with, or simultaneously with the sample is provided with reagent to form a sample zone in the form of a reproduceable gradient. Said sample zone is led into a measuring cell whereupon the flow is stopped by means of shunting the carrier flow, by stopping the pump, or by means of a valve. The reaction is allowed to take place in the measuring cell while a magnitude, characteristic for the reaction, is registered. The reaction speed can be detected from the incline of the obtained response curve and the amount of the sought-after compounds is calculated. By means of setting the stop at a certain point in the concentration gradient curve and always selecting said point at the same time distance from the peak of the gradient curve during flow, a total reproduceability of the analysis is ensured.
REFERENCES:
patent: 3690833 (1972-09-01), Ferrari
patent: 3915644 (1975-10-01), Walraven
patent: 4022575 (1977-05-01), Hansen et al.
patent: 4224033 (1980-09-01), Hansen et al
patent: 4315754 (1982-02-01), Ruzicka et al.
Hansen et al., "Flow Injection Analysis, etc.," Analytica Chimica Acta, 89(1977), pp. 241-254.
Hansen Elo H.
Ruzicka Jaromir
BIFOK AB
Wyse Thomas G.
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