Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1982-09-13
1985-12-31
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 61, 536 63, 26023955A, C07J 1700, C07J 1900
Patent
active
045622507
ABSTRACT:
Methods for the production of steroids (sapogenins) and steroidal glycosides (saponins) from cell, root-cell and shoot tissue cultures of the Yucca plant. The cultures were established and maintained in a suitable nutrient plant cell medium supplemented with growth regulators. The desired products are extracted from the cultures using conventional extraction techniques. Greater total production of steroidal glycosides occurred in root-cell, cell, and shoot tissue cultures in that order. Although the cultures may be grown under conditions ranging from total darkness to total light, steroidal glycoside production was highest in root-cell cultures grown continuously in the light as compared to those grown in the dark. Root-cell culture senescence also enhanced the yield of total steroidal glycosides. Steroids are obtained from the steroidal glycosides by acid hydrolysis to remove the sugar moieties. The production of radio-labeled steroids and steroidal glycosides is disclosed.
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MacCarthy Jean J.
Staba E. John
Brown Johnnie R.
Regents of the University of Minnesota
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