Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-07-22
1994-12-27
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 4351723, 536 231, 536 2431, 530326, 530327, 530350, 530828, C12Q 168
Patent
active
053765303
ABSTRACT:
A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).
REFERENCES:
Petkovich et al., "A Human Retinoic Acid Receptor Which Belongs to the Family of Nuclear Receptors" Nature, vol. 330, 444-450, Dec. 3, 1987.
Benbrook et al., Nature, vol. 333, pp. 669-672, Jun. 16, 1988.
Evans et al, Science, vol. 240, pp. 889-895, May 13, 1988.
Brand et al, Nature, vol. 332, pp. 850-853, Apr. 28, 1988.
DeJean et al. Nature, vol. 372, pp. 70-72, Jul. 3, 1986.
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Brand Nigel
Chambon Pierre
De The Hughes B.
DeJean Anne
Krust Andree
Institut Pasteur
Nucker Christine M.
Scheiner Laurie
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