Steroid secreting human adrenocortical carcinoma cell lines

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

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435363, 435325, 435 701, 435 52, 435 41, C12N 500, C12P 2104, C12P 3300, C12P 100

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057926573

ABSTRACT:
Continuous cell lines have been established from adrenocortical corcinomas. The cell lines are maintained in fully defined serum-free, steroid-free mediums. The cells of the invention, as exemplified by NCI-H295 cells, express all of the major pathways of steroid-ogenesis, including formation of corticosteroids, mineralocorticoids and androgens.

REFERENCES:
A. Leibovitz, et al.; "Brief Communication: New Human Cancer Cell Culture Lines. I. SW-13, Small-Cell Carcinoma of the Adrenal Cortex", J. Natl. Cancer Inst. 51: 691-97 (1973).
B. Fang; PNAS, USA 74: 1067 (1977).
C. Moffett, Fed. Proc. 46: 648 (1987).
D. ATCC Catalogue of Cell Lines & Hybridomas, 6th ed. pp. 63-64 (1988).
E. Staels, et al.; "Regulation of Steroidogenesis in NCI-H295 Cells: A Cellular Model of the Human Fetal Adrenal" Mol. Endo 7(3): 423-433 (1993).
F. Rainey, et al.; "Regulation of Human Adrenal Carcinoma Cell (NCI-H295) Production of C.sub.19 Steroids" J. Clin. Endo Metab 77(3): 731-737 (1993).
G. Bird, et al.; "Human NCI-H295 Adrenocortical Carcinoma Cells: A Model for Angiotensin-II Responsive Aldosterone Secretion" Endo 133(4): 1555-1561 (1993).
H. Murray, et al.; "Gap Junction Assembly and Endocytosis Correlated with Patterns of Growth in a Cultured Adrenocortical Tumor Cell (SW-13)" Cancer Res 41: 4063-4074 (Oct. 1981).
I. Alberts, et al.; Molecular Biology of the Cell pp. 335-339 (1983).

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