Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1991-11-18
1995-06-06
Parr, Margaret
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 6, 435 691, 4352402, 435243, 4352523, 4353201, 536 231, 536 232, 536 234, 536 235, 536 243, 536 2431, 536 2433, 935 3, 935 6, 935 8, 935 9, 935 11, 935 14, 935 23, 935 66, 935 77, 935 78, C07H 2102, C07H 2104
Patent
active
054222621
ABSTRACT:
Disclosed are methods and compositions for the preparation of steroid 5.alpha.-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. Biochemical and pharmacological evidence is presented to demonstrate the existence of more than one human steroid 5.alpha.-reductase. The DNA sequence encoding steroid 5.alpha.-reductase 2, the major active isozyme of human genital tissue, is disclosed herein, in addition to methods and compositions for its preparation and pharmacological analysis. The sequences disclosed herein may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources. These sequences may prove useful in an analysis of normal and abnormal sexual differentiation, benign prostatic hyperplasia, male pattern baldness, acne, hirsutism, endometriosis, and cancer of the prostate.
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Andersson Sefan
Russell David W.
Board of Regents , The University of Texas System
Marschel Ardin H.
Parr Margaret
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