Sterilization indicator with chemically stabilized enzyme

Details Sterilization indicator with chemically stabilized enzyme Sterilization indicator with chemically stabilized enzyme

2005-05-24

2005-05-24

Gitomer, Ralph (Department: 1651)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S031000, C435S288500

Reexamination Certificate

active

06897059

ABSTRACT:
A sterilization indicator for testing the effectiveness of a sterilization procedure comprises a source of an enzyme, a sterilant-resistant chemical associated with the enzyme, and a substrate that reacts with the enzyme to form a detectable enzyme-modified product that provides an indication of the failure of the sterilization procedure. The sterilant-resistant chemical may be a polyglycerol alkyl ester, polyglycerol alkyl ether, an ethoxylated polyhydric alcohol ester, or a polyhydric alcohol ether. The indicator may be used to test the effectiveness of a hydrogen peroxide plasma sterilization procedure and may be provided with a non-challenge test pack or a lumen-challenge test pack.

REFERENCES:
patent: 3239429 (1966-03-01), Menolasino et al.
patent: 3440144 (1969-04-01), Andersen
patent: 3661717 (1972-05-01), Nelson
patent: 4115068 (1978-09-01), Josyln
patent: 4145186 (1979-03-01), Andersen
patent: 4240926 (1980-12-01), McNeely
patent: 4304869 (1981-12-01), Dyke
patent: 4580682 (1986-04-01), Gorski et al.
patent: 4594223 (1986-06-01), Dyke et al.
patent: 4596696 (1986-06-01), Scoville, Jr.
patent: 4636472 (1987-01-01), Bruso
patent: 4642165 (1987-02-01), Bier
patent: 4643876 (1987-02-01), Jacobs et al.
patent: 4650479 (1987-03-01), Insley
patent: 4692307 (1987-09-01), Bruso
patent: 4699765 (1987-10-01), Hambleton
patent: 4739881 (1988-04-01), Bruso
patent: 4756882 (1988-07-01), Jacobs et al.
patent: 4797255 (1989-01-01), Hatanaka et al.
patent: 4828797 (1989-05-01), Zwarun et al.
patent: 4839291 (1989-06-01), Welsh et al.
patent: 4863688 (1989-09-01), Schmidt et al.
patent: 4883641 (1989-11-01), Wicks et al.
patent: 4914034 (1990-04-01), Welsh et al.
patent: 315600 (1991-03-01), Niven
patent: 5057433 (1991-10-01), Douglas
patent: 5064576 (1991-11-01), Suto
patent: 5073488 (1991-12-01), Matner et al.
patent: 5084239 (1992-01-01), Moulton et al.
patent: 5115166 (1992-05-01), Campbell
patent: 5178829 (1993-01-01), Moulton et al.
patent: 5184046 (1993-02-01), Campbell
patent: 5217901 (1993-06-01), Dyckman
patent: 5223401 (1993-06-01), Foltz et al.
patent: 5252484 (1993-10-01), Matner et al.
patent: 5270217 (1993-12-01), Dyke
patent: 5389336 (1995-02-01), Childers
patent: 5405580 (1995-04-01), Palmer
patent: 5418167 (1995-05-01), Matner et al.
patent: 5478749 (1995-12-01), Dyke
patent: 5482684 (1996-01-01), Martens et al.
patent: 5486459 (1996-01-01), Burnham et al.
patent: 5500184 (1996-03-01), Palmer
patent: 5516648 (1996-05-01), Malchesky et al.
patent: 5552320 (1996-09-01), Smith
patent: 5620656 (1997-04-01), Wensky et al.
patent: 5667753 (1997-09-01), Jacobs et al.
patent: 5674450 (1997-10-01), Lin et al.
patent: 5739004 (1998-04-01), Woodson
patent: 5750184 (1998-05-01), Imburgia
patent: 5770393 (1998-06-01), Dalmasso et al.
patent: 5785934 (1998-07-01), Jacobs et al.
patent: 5788941 (1998-08-01), Dalmasso et al.
patent: 5795730 (1998-08-01), Tautvydas
patent: 5801010 (1998-09-01), Falkowski et al.
patent: 5830683 (1998-11-01), Hendricks et al.
patent: 5856118 (1999-01-01), Dalmasso
patent: 5866356 (1999-02-01), Albert et al.
patent: 5872004 (1999-02-01), Bolsen
patent: 5895627 (1999-04-01), Khachatoorian
patent: 5916816 (1999-06-01), Read
patent: 5942438 (1999-08-01), Antonoplos et al.
patent: 6063631 (2000-05-01), Ignacio
patent: 6105761 (2000-08-01), Peuker et al.
patent: 6287518 (2001-09-01), Ignacio et al.
patent: 6346417 (2002-02-01), Ignacio et al.
patent: 6355448 (2002-03-01), Foltz et al.
patent: 6485971 (2002-11-01), Kaur et al.
patent: 6485978 (2002-11-01), Kirckof et al.
patent: 6485979 (2002-11-01), Kippenhan et al.
patent: 6488890 (2002-12-01), Kirckof
patent: 6566090 (2003-05-01), Witcher et al.
patent: 6623955 (2003-09-01), Matner et al.
patent: 20020037546 (2002-03-01), Hendricks et al.
patent: 20020051733 (2002-05-01), Antonoplos et al
patent: 20020058296 (2002-05-01), Miller et al.
patent: 20030077688 (2003-04-01), Matner et al.
patent: 20030157588 (2003-08-01), Matner et al.
patent: 20030215923 (2003-11-01), Witcher et al.
patent: 676743 (1994-12-01), None
patent: 0 254 428 (1988-01-01), None
patent: 0 255 229 (1988-02-01), None
patent: 0 419 282 (1991-03-01), None
patent: 0 421 760 (1991-04-01), None
patent: 0 421 760 (1994-03-01), None
patent: 0 638 650 (1995-02-01), None
patent: 0 707 186 (1996-04-01), None
patent: 62115266 (1987-05-01), None
patent: 62205748 (1987-09-01), None
patent: 62205750 (1987-09-01), None
patent: 62253357 (1987-11-01), None
patent: 63079579 (1988-04-01), None
patent: 63275517 (1988-11-01), None
patent: WO 9428164 (1994-12-01), None
patent: WO 9726924 (1997-07-01), None
patent: WO 9846994 (1998-10-01), None
patent: WO 0110473 (2001-02-01), None
patent: WO 0140792 (2001-06-01), None
Alfa et al., “Comparison of Ion Plasma, Vaporized Hydrogen Peroxide, and 100% Ethylene Oxide Sterilizers to the 12/88 Ethylene Oxide Gas Sterilizer”,Infection Control and Hospital Epidemiology,vol. 17, No. 2, Feb. 1996, pp. 92-100.
Baker, et al., “Thermal Stabilization of Fungal β-Glucosidase through Glutaraldehyde Crosslinking,”Biotechnology Letters,vol. 10, No. 5, 325-330 (1988).
Barbaric, et al., “Stabilization of Glycoenzymes by Cross-linking of Their Carbohydrate Chains,”Annals New York Academy of Sciences,vol. 542, pp. 173-179 (1988).
Corry, J., “The Effect of Sugars and Polyols on the Heat Resistance of Salmonellae,”J. Appl. Bact.,vol. 37, pp. 31-43 (1974).
Gottschalk, et al., “Chemically Crosslinked Lactate Dehydrogenase: Stability and Reconstitution after Glutaraldehyde Fixation,”Biotechnology and Applied Biochemistry,vol. 9, No. 5, pp. 389-400 (Oct. 1987).
Hoshino, et al., “A study on the thermostability of microencapsulated glucose oxidase,”J. Microencapsulation,vol. 6, No. 2, pp. 205-211 (1989).
Ichiba, et al., “Cation-induced thermostability of yeast andEscherichia colipyrophosphatases,”Biochem. Cell Biol.,vol. 66, pp. 25-31 (Jan. 1988).
Kokufuta, et al., “Useful of Polyelectrolyte Complex-Stabilized Calcium Alginate Gel for Entrapment of β-Amylase”,Biotechnology and Bioengineering,vol. 32, pp. 756-759 (1988).
Laurence, “Fluorescence Techniques for the Enzymologist”,Methods in Enzymology,vol. 4, S. P. Colowick and N.O. Kaplan, Eds.,Academic Press,New York, 1957.
Lenders, et al. “Chemical Stabilization of Glucoamylase fromAspergillus nigeragainst Thermal Inactivation,”Biotechnology and Bioengineering,vol. 31, pp. 267-277 (1988).
Leonowicz, et al., “Improvement in stability of an immobilized fungal laccase,”Applied Microbiology and Biotechnology,vol. 29, No. 2-3, pp. 129-135 (1988).
Mecke, “Hydrogen Peroxide Plasma—an Interesting Microbiocidal Concept”, Hygiene + Medizin, 1992:17:pp. 537-543.
Olsen, et al. “Improvement of bacterial β-glucanase thermostability by glycosylation,”Journal of General Microbiology,vol. 137, pp. 579-585 (1991).
Redway, et al., “Effect of Carbohydrates and Related Compounds on the Long-Term Preservation of Freeze-Dried Bacteria,”Cryobiology,vol. 11, pp. 73-79 (1974).
Roth, “Fluorimetric Assay of Enzymes”,Methods of Biochemical Analysis,vol. 17, D. Block, Ed.,Interscience Publishers,New York, 1969.
Rutala et al., “Comparative evaluation of the sporicidal activity of new low-temperature sterilization technologies: Ethylene oxide, 2 plasma sterilization systems, and liquid peracetic acid”,AJIC,vol. 26, No. 4, Aug. 1998, pp. 393-398.
Smith, et al., “Effect of Environmental Conditions during Heating on Commercial Spore Strip performance,”Applied and Environmental Microbiology,vol. 44, No. 1, pp. 12-18 (Jul. 1982).
Srivastava, R., “Studies on stabilization of amylase by covalent coupling to soluble polysaccharides,”Enzyme Microb. Technol.,vol. 13, No. 2, pp. 164-170 (Feb. 1991).
Srivastava, R., “Effect of glycosylation of bacterial amylase in stability and active site conformation,”Indian Journal of Biochemistry&Biophysics,vol

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