Sterile flow cytometer and sorter with mechanical isolation betw

Details Sterile flow cytometer and sorter with mechanical isolation betw Sterile flow cytometer and sorter with mechanical isolation betw

1997-02-20

1998-07-07

Snay, Jeffrey

Chemistry: analytical and immunological testing

Biological cellular material tested

436164, 422 33, 422 8201, 422 8205, 422295, 356 72, 356 73, 2504612, 209 31, G01N 2900

Patent

active

057767815

ABSTRACT:
An apparatus and process for measuring particles that prevents the particles from being contaminated during the measuring and optional sorting process wherein a flow chamber or region is mechanically isolated from a sterile enclosure which surrounds the flow chamber. The present invention in one embodiment provides an apparatus comprising of a droplet generator, a charging system, a deflecting system, a sterilization system, a light source, a detector, a cooling system and a vacuum system. The droplet generator and the charging system and the flow chamber associated with the droplet generator operate within an illumination frame where the particles (e.g. biological cells) are actually measured. In order to prevent the biological cells from being contaminated, the illumination frame is located within an isolation chamber which provides an environment that can be sterilized before and after the actual measuring and sorting of the cells, but the illumination frame is mechanically isolated from the isolation chamber. The sterilization system, located outside the isolation chamber sterilizes the isolation chamber before cells are measured and sorted within the isolation chamber, and furthermore, ensures that the isolation chamber is maintained at a pressure above (or alternatively, below) atmospheric pressure during the measuring and sorting of the biological cells. The illumination frame, the light source and the detector are supported by a table to enable the light beam(s) from the light source to intersect the biological cells flowing through the illumination frame, thereby emitting flashes of light (or otherwise effecting the light beam) which are detected by the detector.

REFERENCES:
patent: 3560754 (1971-02-01), Kamentsky
patent: 3710933 (1993-01-01), Fulwyler et al.
patent: 4111753 (1978-09-01), Folsom
patent: 4230031 (1980-10-01), Pedroso et al.
patent: 4304224 (1981-12-01), Fortney
patent: 5083558 (1992-01-01), Thomas et al.
patent: 5150313 (1992-09-01), van den Engh et al.
patent: 5200616 (1993-04-01), Kokawa et al.
patent: 5464581 (1995-11-01), van den Engh
Melamed, M.R. et al. An Historical Review of the Developiment of flow Cytometers and Sortes, in: Flow Cytometry and Sorting, Second Edition (Wiley-Liss Inc., 1990), pp. 1-9.
Steen, H.B. Charactioristice of Flow Cytometers, in: Flow Cytometry and Sorting, Second Edition (Wiley-Liss Inc., 1990), pp. 11-25.
Lindmo, T. et al. flow Sorters for Biological Cells, in: Flow Cytometry and Sorting, Second Edition (Wiley-Liss Inc., 1990), pp. 145-169.
Dean, P.N. Commericial Instruments, in: Flow Cytometry and Sorting, Second Edition (Wiley-Liss Inc., 1990), pp. 171-186.

Affiliated with

Also associated with

Rating

Sterile flow cytometer and sorter with mechanical isolation betw does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sterile flow cytometer and sorter with mechanical isolation betw, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sterile flow cytometer and sorter with mechanical isolation betw will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1204828