Steric hindrance enzyme immunoassay

Details Steric hindrance enzyme immunoassay Steric hindrance enzyme immunoassay

1980-10-15

1982-04-06

Wiseman, Thomas

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 424 8, 424 12, 435188, 435810, G01W 3354, C12W 996

Patent

active

043236472

ABSTRACT:
A novel separation technique is described that is particularly useful for effecting separations in enzyme immunoassay procedures. A mixture, in an aqueous liquid vehicle, of (1), ligand-enzyme conjugate, and of (2), the conjugate bound through its ligand moiety to a receptor, is brought into contact with an insoluble, immobilized pseudo-substrate material, to which the enzyme normally binds. Free conjugate binds and becomes insoluble. Bound conjugate remains in the liquid phase. The ligand may be an antigen and the receptor, the antibody to the antigen.
This separation technique makes feasible several sensitive immunoassay procedures. The material to be assayed may be, for example, rubella virus; hepatitis B surface antigen; gonorrhea antigen; the antibody to any of the foregoing; a general antibody, i.e., an immunoglobulin; a hormone such as choriomammotropin; a steroid, hapten, or the like.

REFERENCES:
patent: Re29169 (1977-04-01), Schuurs et al.
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 3666421 (1972-05-01), Price
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 3875011 (1975-04-01), Rubenstein et al.
patent: 3879262 (1975-04-01), Schuurs et al.
patent: 3880715 (1975-04-01), Schneider
patent: 3905871 (1975-09-01), Rubenstein et al.
patent: 3935074 (1976-01-01), Rubenstein et al.
patent: 4016043 (1977-04-01), Schuurs et al.
patent: 4190496 (1980-02-01), Rubenstein et al.
patent: 4203802 (1980-05-01), Rubenstein et al.
patent: 4213764 (1980-07-01), O'Connor
patent: 4233401 (1980-11-01), Yoshida et al.
patent: 4273866 (1981-06-01), Voss et al.
patent: 4279992 (1981-07-01), Bougaslaski et al.
Monji, et al., "Steric Hindrance Enzyme Immunoassay (SHEIA), A Novel Method in Enzyme Immunoassay", Res. Comm. Chem. Path. Pharm, vol. 26, No. 1, (1979), pp. 187-196.
Steers et al., "B-Galactosidase", Methods of Enzymology, vol. XXXIV Part B, Academic Press, N.Y. (1974), pp. 350-358.
Daughaday et al., "Methods of Separating Antibody-Bound From Free Antigen", Principles of Competitive Protein-Binding Assays, Odell et al., ed., J. B. Lippincott Co., Philadelphia, pp. 303-324.
Castro, et al., "Automated Radio Immunoassay of Choriomammotropin (Human Placental Lactogen)", Clin. Chem. vol. 22, No. 10, (1976) pp. 1655-1658.
Wisdom "Enzyme-Immunoassay", Clinical Chem. 22, 1243-1255 (1976).
Skelley, "Radioimmunoassay", Clinical Chem. 19, 146-186, (1973).
Schuurs et al., Clinical Chem. Acta 81, 1-40 (1977).
Ratcliff, "Separation Techniques in Saturation Analysis", Br. Med. Bull. 30, 32-37 (1974).
Dray et al., Biochem. Biophys. Acta. 403, 131-138 (1975).
McEwen et al., Nature 226, 263 (1970).
Vonderbaar et al., Biochem. Biophys. Acta. 176, 626 (1969).
Exley et al., FEBS Letters 79, 301-304 (1977).
Dittmar et al., Clinical Chem. 25, 227-229 (1979).
Kitagawa and Aikawa, J. Biochem. 79, 233-236 (1976).
Murphy et al., J. Clin. Endocr. 28, 343 (1968).
Murphy et al., J. Clin. Endocr. 27, 973 (1967).
Murphy et al., J. Clin. Endocr. 24, 187 (1964).
Steers et al., Methods in Enzymology 34, 350-358 (1974).
FEBS Letters 91, 162-165 (1978).
Nature 219, 168 (1968).
Page et al., J. Clin. Endocr. 28, 200 (1969).
Exley et al., Steriods 18, 593 (1971).
Rubenstein et al., "Homogenous Enzyme Immunoassay a New Immunochemical Technique", Biochem. Biophys. Research Comm. 47, 846-851 (1972).
Yalow and Berson, J. Clin. Invest. 39, 1157 (1960).

Affiliated with

Also associated with

Rating

Steric hindrance enzyme immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Steric hindrance enzyme immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Steric hindrance enzyme immunoassay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-593120