Stereospecific asparaginases

Details Stereospecific asparaginases Stereospecific asparaginases

1981-04-10

1984-09-25

Meyers, Albert T.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, 435253, C12N 982, C12N 120

Patent

active

044736460

ABSTRACT:
There is described a method of separating an asparaginase rich fraction from a thermophilic microoganism and then to divide this fraction into its D- and L-asparaginases. Substantially pure crystals of the D-asparaginase were obtained.

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Hickey et al., The Electron Transport System of an Extremely Thermophlic Bacterium, J. of Gen. Micro., 1979, 114, pp. 195-200.
Degryse, et al., A Comparative Analysis of Extreme Thermophilic Bacteria Belonging to the Genus Thermus Chem. Abs. vol. 89, 1978, p. 420.
Brock, et al., Thermus aquaticus gen. n. and sp. n., a Non-sporulating Extreme Thermophilie, J. of Bacteriology, vol. 98, No. 1, pp. 289-297 1969.
Watanabe, et al., Thermophilic L-asparginase Chem Abstracts vol. 86, 1977 p. 424.
"The Purification and Some Properties of a Stereospecific D-Asparaginase from an Extremely Thermophilic Bacterium, Thermus aquaticus" Graeme R. Guy and R. M. Daniel; 1982.
"Stereospecific Asparginases in Smooth Brucella abortus, Strain 19" Robert A. Altenbern and Riley D. Housewright, Aug. 21, 1953.
"Geology of Whakarewarewa Hot Springs:, E. F. Lloyd, 1975.

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