Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Reexamination Certificate
2011-06-07
2011-06-07
Ton, Thaian N (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
Reexamination Certificate
active
07955850
ABSTRACT:
In this invention is described a method that foresees the isolation of a new subpopulation of stem cells derived form dental pulp, whose differentiation is osteoblasts lead to the subsequent production and employment of a bone tissue, called LAB (Living Autologous Bone). Specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and of dental germs, obtained from human subjects; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular sub-population, which, after differentiation in osteoblasts, is able to produce in vitro an extracellular matrix, identical to that detectable in bone tissue; 3) the use of this selected and differentiated cell population in order to produce autologous bone tissue in vitro, containing vital osteoblasts; 4) the preservation of the LAB under conditions which guarantee cellular vitality; 5) the use of the LAB in donor patients to reconstruct bone tissue, as required in the daily practice in dentistry, maxillo-facial surgery and orthopedics.
REFERENCES:
patent: 5306305 (1994-04-01), Lee
patent: 2004/0058442 (2004-03-01), Shi et al.
patent: 2007/0274958 (2007-11-01), Shi et al.
Shi et al., “Perivascula Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental Pulp”, J Bone Min Res 18(4):696-704 (2003).
Gronthos et al., “Postnatal Human Dental Pulp Stem Cells (DPSCs) in vitro and in vivo”, Proc Nat Acad Sci US 97(25):13625-13630 (2000).
Tsukamoto et al., “Mineralized Nodula Formation by Cultures of Human Dental Pulp-Derived Fibroblasts”, Archs Oral Biol 37(12):1045-1055 (1992).
Laino et al., “An Approchable Human Adult Stem Cell Source for Hard-Tissue Engineering”, J Cell Physiol 206:693-701 (2006).
Laino et al., “A New Population of Human Adult Dental Pulp Stem Cells: A Useful Source of Living Autologous Fibrous Bone Tissue (LAB)”, J Bone Min Res 20(8):1394-1402 (2005).
Miura et al., “SHED: Stem Cells from Human Exfolidated Deciduous Teeth”, Proc Nat Acad Sci US 100(10):5807-5812 (2003).
Carinci Francesco
D'Aquino Riccardo
De Rosa Alfredo
Graziano Antonio
Laino Gregorio
Porzio, Bromberg & Newman P.C.
Ton Thaian N
LandOfFree
Stem cells obtained from pulp of deciduous or permanent... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stem cells obtained from pulp of deciduous or permanent..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stem cells obtained from pulp of deciduous or permanent... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2717411