Stem cell-specific promoters and their use

Details Stem cell-specific promoters and their use Stem cell-specific promoters and their use

2008-07-08

2008-07-08

Wehbe, Anne Marie S. (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C435S325000

Reexamination Certificate

active

07396680

ABSTRACT:
The present invention relates to a method of isolating stem cells from a mixed population of different cell types. This method involves selecting a promoter which functions only in said stem cells and not in the other cell types. The promoter can have a nucleotide sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8. A nucleic acid molecule encoding a marker protein under control of said promoter is introduced into all cell types of said mixed population and only the stem cells, but not the other cell types, within said mixed population are allowed to express said marker protein. The cells of said mixed population of cell types that are expressing the marker protein, which are restricted to the stem cells are identified and separated from the mixed population, where the separated cells are restricted to the stem cells. The promoter, nucleic acid constructs comprising it, and the resulting stem cells are also disclosed. The nucleic constructs can be incorporated in transgenic animals in order to monitor stem cell movement. A disorder mediated by stem cell proliferation can be carried out by administering both (1) a nucleic acid construct comprising the promoter of the present application and an enzyme capable of converting a prodrug to a cytotoxic drug and (2) the prodrug.

REFERENCES:
Corbeil et al. J. Biol. Chem. 275(8):5512-5520; 2000.
Yu et al. J. Biol. Chem. 2777(23):20711-20716; 2002.
GenBank Accession No. AY275524.
GenBank AC108063 pp. 1-63; Mar. 9, 2002.
Miraglia et al., “A Novel Five-Transmembrane Hematopoietic Stem Cell Antigen: Isolation, Characterization, And Molecular Cloning,” Blood, 90:5013-5021 (1997).
Yin et al., “AC133, A Novel Marker For Human Hematopoietic Stem And Progenitor Cells,” Blood, 90:5002-5012 (1997).
Peichev et al., “Expression Of VEGFR-2 And AC133 By Circulating Human CD34+ Cells Identifies A Population Of Functional Endothelial Precursors,” Blood, 95:952-958 (2000).
Salven et al., “VEGFR-3 And CD133 Identify A Population Of CD34+ Lymphatic/Vascular Endothelial Precursor Cells,” Blood, 101:168-172 (2003).
Uchida et al., “Direct Isolation Of Human Central Nervous System Stem Cells,” Proc. Natl Acad. Sci. USA, 97:14720-14725 (2000).
Lee et al., “Isolation Of Neural Stem Cells From The Postnatal Cerebellum,” Nature Neuroscience, 8:723-729 (2000).
Richardson et al., “CD133, A Novel Marker For Human Prostatic Epithelial Stem Cells,” J. Cell Sci., 117:3539-3545 (2004).
Singh et al., “Identification Of A Cancer Stem Cell In Human Brian Tumors,” Cancer Research, 63:5821-5828 (2003).
Singh et al., “Identification Of Human Brain Tumour Initiating Cells,” Nature, 432:396-401 (2004).
Rafii et al., “Therapeutic Stem And Progenitor Cell Transplantation For Organ Vascularization And Regeneration,” Nature Medicine, 9:1-11 (2003).
Heissig et al., “Recruitment Of Stem And Progenitor Cells From The Bone Marrow Niche Requires MMP-9 Mediated Release of Kit-Ligand,” Cell, 109:625-637 (2002).
Kim et al., “The Emerging Fields Of Suicide Gene Therapy And Virotherapy,” Trends In Molecular Medicine, 8:68-73 (2002).
Yazawa et al., “Current Progress In Suicide Gene Therapy For Cancer,” World Journal Of Surgery, 26:783-789 (2002).
Denny, “Prodrugs For Gene-Directed Enzyme-Prodrug Therapy (Suicide Gene Therapy),” Journal Of Biomedicine And Biotechnology, 2003:48-70 (2003).

Affiliated with

Also associated with

Rating

Stem cell-specific promoters and their use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Stem cell-specific promoters and their use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stem cell-specific promoters and their use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2812802