Stem cell populations and methods of use

Details Stem cell populations and methods of use Stem cell populations and methods of use

2011-01-04

2011-01-04

Belyavskyi, Michail A (Department: 1644)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

Reexamination Certificate

active

07863043

ABSTRACT:
Populations of stem cells and methods for their isolation and use are provided. These stem cell populations comprise aldehyde dehydrogenase positive (ALDHbr) cells isolated from bone marrow, and ALDHbrCD105+cells derived from any stem cell source. These populations may also comprise cells expressing such surface markers as CD34, CD38, CD41, CD45, CD105, CD133, CD135, CD117, and HLA-DR, and/or are substantially free from such cell surface markers as CD3, CD7, CD 10, CD 13, CD 14, C1319, CD33, CD35, CD56, CD 127, CD 138, and glycophorin A. The population may also comprise cells expressing CD90. The stem cell populations of the invention are isolated from a stem cell source such as bone marrow, peripheral blood, umbilical cord blood, and fetal liver. Methods of the invention comprise isolating and purifying stem cell populations from stem cell sources, and methods of using these cells to reconstitute, repair, and regenerate tissues.

REFERENCES:
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5876956 (1999-03-01), Jones et al.
patent: 1 302 534 (2003-04-01), None
patent: WO99/02654 (1999-01-01), None
patent: WO 00/34507 (2000-06-01), None
patent: WO 02/36751 (2002-05-01), None
patent: WO 02/36751 (2002-05-01), None
Barry et al. (1999) “The Monoclonal Antibody SH-2 Raised Against Human Mesenchymal Stem Cells, Recognizes an Epitope on Endoglin (CD105)”Biochem Biophys Res Commun265:134-139.
Majumdar et al. (2000) “Isolation, Characterization, and Chondrogenic Potential of Human Bone Marrow-Derived Multipotential Stromal Cells”J Cell. Physiol. 185:98-106.
Pittenger et al. (1999) “Multilineage Potential of Adult Human Mesenchymal Stem Cells”Science284:143-147.
Cai, J., et al., “Membrane Properties of Rat Embryonic Multipotent Neural Stem Cells,”Journal of Neurochemistery, 2004, pp. 212-226, vol. 88.
Christ, O., et al., “Short and Longterm Repopulating Cells in Human Cord Blood Display Different Levels of Aldehyde Dehydrogenase Activity as Revealed by Assays of BODIPY-Stained Cells in NOD/SCID Mice,”Blood, 2003, vol. 102(11), Abstract No. 1178.
Fallon, P., et al., “Mobilized Peripheral Blood SSCloALDHbrCells Have the Phenotypic and Functional Properties of Primitive Haematopoietic Cells and Their Number Correlates With Engraftment Following Autologous Transplantation,”British Journal of Haematology, 2003, pp. 99-108, vol. 122.
Fiordalisi, M., et al., “Surface Phenotype of Bone Marrow Cells That Express High Levels of Aldehyde Dehydrogenase (ALDH),”Blood, 2003, vol. 102(11), Abstract No. 4302.
Foster, S., et al., “Specificity of the Flow Cytometric Aldehyde Dehydrogenase [ALDH] Assay in Hematopoietic Progenitor Cells and Tumor Cell Lines,”Blood, 2003, vol. 102(11), Abstract No. 3580.
Gentry, T., et al., “Aldehyde Dehydrogenase [ALDH] and Surface Antigen Expression Define Hematopoeitic Stem and Progenitor Cell [HSPC] Subsets Differentially Represented in Mobilized Peripheral Blood [PBSC], Umbilical Cord Blood [UCB], and Bone Marrow [BM],”2004 Tandem BMT Meetings, File name: 150245.
Hess, D., et al., “Functional Characterization of Highly Purified Human Hematopoietic Repopulating Cells Isolated Based on Aldehyde Dehydrogenase Activity,”Blood, 2003, vol. 102(11), Abstract No. 383.
Lodie, T., et al., “Systematic Analysis of Reportedly Distinct Populations of Multipotent Bone Marrow-Derived Stem Cells Reveals a Lack of Distinction,”Tissue Engineering, 2002, pp. 739-751, vol. 8(5).
Meyerrose, T., et al., “Isolation of Human Aldehyde Dehydrogenase—Expressing Stem Cells; A Population with Increased Homing and Hematopoietic Potential,”Blood, 2003, vol. 102(11), Abstract No. 1175.
Storms, R., et al., “Isolation of Primitive Human Hematopoietic Progenitors on the Basis of Aldehyde Dehydrogenase Activity,”Proc. Natl. Acad. Sci. USA, 1999, pp. 9118-9123, vol. 96.
Verfaillie, C., “Adult Stem Cells: Assessing the Case for Pluripotency,”Trends in Cell Biology, 2002, pp. 502-508, vol. 12(11).

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