STED-fluorescent light microscopy with two-photon excitation

Details STED-fluorescent light microscopy with two-photon excitation STED-fluorescent light microscopy with two-photon excitation

2011-01-04

2011-01-04

Porta, David P (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

C250S458100

Reexamination Certificate

active

07863585

ABSTRACT:
A method of high spatial resolution imaging a structure in a sample comprises: marking the structure with molecules of a fluorescent dye; selecting a first wavelength for excitation light which excites the molecules of the fluorescent dye via a multi photon process for spontaneous emission of fluorescent light; focusing pulses of the excitation light into the sample to excite those molecules of the fluorescent dye present in a focal area of the focused excitation light; selecting a second wavelength shorter than the first wavelength for de-excitation light which de-excites excited molecules of the fluorescent dye prior to their spontaneous emission; during a plurality of the pulses of the excitation light, continuously directing the de-excitation light onto the sample to de-excite excited molecules of the fluorescent dye, which are located outside an measurement area which is a fraction of the focal area; and recording the fluorescent light spontaneously emitted by the molecules of the fluorescent dye in the sample.

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English Translation of International Preliminary Report on Patentability and Search Report for co-pending, related PCT Application No. PCT/EP2008/060694, mailed Mar. 2, 2010.

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