Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-11-23
1998-05-26
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435239, 435325, 436 63, C12Q 170, C12N 506, C12N 702, G01N 3348
Patent
active
057562814
ABSTRACT:
A method of detecting a stealth virus is provided by culturing a sample under conditions in which any stealth virus in the sample is able to induce a cytopathic effect. A method for culturing a virus is also provided by (a) cocentrifuging a sample of the virus with a permissive cell line of indicator cells; (b) inoculating the cell mixture into culture vessels; (c) adding viral enhancing medium to the culture; and (d) detecting in vitro a cytopathic effect in the permissive cell line.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5426028 (1995-06-01), Levy et al.
Ablashi et al., "Utilization of Human Hematopoietic Cell Lines For the Propagation and Characterization of HBLV (Human Herpesvirus 6)," Int. J. Cancer 42:787-791 (1988).
Archard et al., "Postviral Fatigue Syndrome: Persistance and Enterovirus RNA in Muscle and Elevated Creatine Kinase," The Royal Society of Medicine vol. 81 (1988).
Buchwald et al., "A Chronic Illness Characterized by Fatigue, Neurologic and Immunologic Disorders and Active Human Herpesvirus Type 6 Infection," Annals of Internal Medicine 116:103-113 (1992).
Chee et al., "Analysis of the Protein Coding Content of the Sequence of Human Cytomegalovirus Strain AD169," Current Topics in Microbioloigy and Immunology 154:126-169 (1990).
Cohen et al., "Okadaic Acid: A New Probe for the Study of Cellular Regulation," TIBS 15:98-102 (1990).
Dale et al., "The Inoue-Melnick Virus, Human Herpesvirus Type 6, and the Chronic Fatigue Syndrome," Annals of Internal Medicine 110:92-93 (1989).
Dale et al., "Chronic Fatigue Syndrome: Lack of Association with Hepatitis C Virus Infection," J. Medical Virology 34:119-121 (1991).
DeFreitas et al., "Retroviral sequences related to human T-lymphotropic virus type II in patients with chronic fatigue immune dysfunction syndrome," Chemical Abstracts 114:No. 205331c (1991).
DeFreitas et al., "Retroviral Sequences Related To Human T-lymphotropic Virus Type II in Patients with Chronic Fatigue Immune Dysfunction Syndrome," Proc. Natl. Acad. Sci. USA 88:2922-2926 (1991).
Demitrack et al., "Evidence for Impaired Activation of the Hypothalamic-Pituitary-Adrenal Axis in Patients with Chronic Fatigue Syndrome," Journal of Clinical Endocrinology and Metabolism 73:1224-1234 (1991).
Ehrlich et al., "Detection of Human T-Cell Lymphoma-Leukemia Viruses," PCR Protocols: A Guide to Methods and Applications, Ch. 39, pp. 325-336 (1990).
Gupta and Vayuvegula, "A Comprehensive Immunological Analysis in Chronic Fatigue Syndrome," Scand. J. Immunol. 33:319-327 (1991).
Ham, R.G., "An Improved Nutrient Solution for Diploid Chinese Hamster and Human Cell Lines", Exp. Cell Research 29:515-526 (1963).
Ho, M., Cytomegalovirus: Biology and Infection, 2nd ed., pp. 75-76, Plenum Medical Book Company, New York, N.Y. (1991).
Holmes, G.P. "Defining the Chronic Fatigue Syndrome," Reviews of Infection Diseases 13(Suppl. 1):553-555 (1991).
Holmes et al., "Chronic Fatigue Syndrome: A Working Case Definition," Annals of Internal Medicine 108:387-389 (1988).
Iscove and Melchers, "Complete Replacement of Serum By Albumin, Transferrin and Soybean Lipid in Cultures of Lipopolysaccharide-Reactive B Lymphocytes," J. Exp. Med. 147:923-933 (1978).
Kendall, R.E., "Chronic Fatigue Viruses and Depression," The Lancet 337:160-161 (1991).
Landay et al., "Chronic Fatigue Syndrome: Clinical Condition Associated with Immune Activation," The Lancet 338:707-712 (1991).
Martin, W.J., "Ch. 27--Detection of Viral Related Sequences in CFS Patients Using the Polymerase Chain Reaction," The Clinical and Scientific Basis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome, pp. 278-281, Hyde et al. eds. The Nightingale Research Foundation, Ogdensburg, New York (1992).
Martin, W.J., "Ch. 34--Viral Infekction in CFS Patients," The Clinical and Scientific Basis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome, pp. 325-3271, Hyde et al. eds., The Nightingale Research Foundation, Ogdensburg, New York (1992).
Niks and Otto, "Towards an Optimized MTT Assay," Immunological Methods 130:149-151 (1990).
Palca, J., "Does a Retrovirus Explain Fatigue Syndrome Puzzle?" Science 249:1240-1241 (1990).
Rethwilm et al., "Infectious DNA of the Human spumaretrovirus," Nucleic Acids Research 18:733-738 (1990).
Schirmer et al., "Differentiation Between Two Distinct Classes of Viruses Now Classified as Human Herpesvirus 6," Proc. Natl. Acad. Sci. USA 88:5922-5926 (1991).
Shafran, S.D., "The Chronic Fatigue Syndrome, " The American Journal of Medicine 90:730-738 (1991).
Shepherd, C., "Myalgic Encephalomyeitis--Is It a Real Disease?" The Practitioner 233:41-46 (1989).
Welch et al., "Cytomegalovirus Homologs of Cellular G Protein-Coupled Receptor Genes Are Transcribed," J. Virology 65:3915-3918 (1991).
Yousef et al., "Chronic Enterovirus Infection in Patients with Postviral Fatigue Syndrome," Lancet i:146-150 (1988).
Palca (1991), Science 254: 1726-1728.
Ablashi et al. (1991), Virology 184: 545-552.
Werner et al. (1979), Lancet II : 258-259 (1979).
Freshney (1987), Culture of Animal Cells : A Manual of Basic Technique, Second Ed. (Alan R. Liss, Inc., New York), pp. 127-136.
Ausubel et al. (1992), Short Protocols in Molecular Biology, Second Ed., (John Wiley + Sons, New York) pp. 16-36 to 16-38.
DiLuca et al. (1990), Virology 175:199-210.
Sundin et al. (1989), J. Clin. Microbiol. 27(7): 1659-1660.
Fields et al. (1985), Virology (Raven Press, New York) pp. 427-432.
Gunn et al. (1993), Morbidity and Mortality Weekly Report 42(10): 183, 189, 190.
Cowley et al. (1996), Newsweek, Apr. 22, 1996, p. 62.
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