Static continuous electrophoresis device

Details Static continuous electrophoresis device Static continuous electrophoresis device

1981-10-06

1982-11-09

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180R, G01N 2726, G01N 2728

Patent

active

043583580

ABSTRACT:
Apparatus is disclosed for carrying out a moving wall type electrophoresis process for separation of cellular particles. The Apparatus includes a water-tight housing (10) containing an electrolytic buffer solution. A separation chamber (12) in the housing is defined by spaced opposed moving walls (14 and 16) and spaced opposed side walls (18 and 20). Substrate assemblies (26 and 32), which support the moving wall include vacuum ports (60) for positively sealing the moving walls against the substrate walls. A plurality of suction conduits (62) communicate with the suction ports and are arranged in the form of valleys in a grid plate (64). Raised land portion of the grid plate support the substrate walls against deformation inwardly under suction. Cooling chamber (70) carried on the back side of plate (64). The apparatus also has tensioner means including roller (80) and adjustment screws (82) for maintaining the belts in position and a drive arrangement including an electric motor (90) with a gear (94) affixed to its output shaft. Electrode assemblies (56 and 57) are disposed to provide the required electric field.

REFERENCES:
patent: 3758395 (1973-09-01), Strickler
patent: 3847773 (1974-11-01), Snyder
patent: 4059501 (1977-11-01), Strickler
patent: 4309268 (1982-01-01), Richman
patent: 4310408 (1982-01-01), Rose et al.

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