Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1982-12-08
1985-08-27
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, 435284, 435287, 435311, 435313, 210342, 210645, C12M 112, C12M 104, C12M 300, C12M 100, B07B 130, B07C 516, C12N 500, C12N 502
Patent
active
045378606
ABSTRACT:
A method and apparatus for maintaining animal cells in vitro in a substantially arrested state of proliferation with continuous secretion of cell product is disclosed. The cells are retained within a reactor vessel chamber in a semi-rigid matrix having interstices for passage of fluid nutrient medium. Fresh nutrient medium is supplied by perfusion into the matrix through relatively low porosity tubes which are suspended in the reactor chamber and which substantially traverse the matrix; expended medium and cell product is withdrawn through relatively high porosity tubes which also are suspended in the reactor chamber and which substantially traverse the matrix; and oxygenated gaseous medium is supplied by perfusion into the matrix through a selectively-permeable membrane disposed in the reactor chamber.
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Feder Joseph
Lewis, Jr. Charles
Tolbert William R.
Meyer Scott J.
Monsanto Company
Teskin Robin
Williams, Jr. James W.
Wiseman Thomas G.
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