Starch sponge column apparatus and process for immobilized enzym

Details Starch sponge column apparatus and process for immobilized enzym Starch sponge column apparatus and process for immobilized enzym

1976-08-23

1977-07-05

Shapiro, Lionel M.

Textiles: fiber preparation

195 31F, 195 63, 195 68, 195116, 195139, 195DIG11, C12D 1302, C12B 100, C07G 702

Patent

active

040338207

ABSTRACT:
A column apparatus and process is provided for immobilized enzyme reactions using a bed of starch in sponge form, the starch having been chemically modified to incorporate enzyme-immobilzing groups. The starch sponge bed of the column apparatus has a flow of direction length of 2 to 5 feet or more, and a water flow porosity such that over 0.5 (preferably over 1.0) gal.water/min.ft..sup.2 flows through the bed. The apparatus and process can be used with a wide variety of enzymes. It has applicability to the enzymatic conversion of starch hydrolysates, since the starch forming the sponge bed can be cross-linked to increase its resistance to enzyme attack. Starch-derived sugar syrups having D.E.'s of 40 to 97 can be produced. Also, isomerization of glucose syrups can be carried out to produce a syrup of enhanced sweetness, comprising a mixture of glucose and fructose.

REFERENCES:
patent: 3919048 (1975-11-01), Dahlmans et al.
patent: 3944470 (1976-03-01), Diehl et al.
patent: 3960663 (1976-06-01), Tamura et al.

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