Starch debranching enzymes

Details Starch debranching enzymes Starch debranching enzymes

2008-05-05

2010-10-19

Raghu, Ganapathirama (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S200000, C435S183000, C435S098000, C435S096000, C435S069100, C536S023200

Reexamination Certificate

active

07816113

ABSTRACT:
The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isoamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

REFERENCES:
patent: 4335208 (1982-06-01), Norman
patent: 4560651 (1985-12-01), Nielsen et al.
patent: 5352602 (1994-10-01), Yamada et al.
patent: 5486469 (1996-01-01), Antranikian et al.
patent: 6265197 (2001-07-01), Bisgard-Frantzen
patent: 6300115 (2001-10-01), Teague et al.
patent: 6350599 (2002-02-01), Svendsen
patent: 48091272 (1973-11-01), None
patent: WO 95/23850 (1995-09-01), None
patent: WO 95/23852 (1995-09-01), None
patent: WO 95/23853 (1995-09-01), None
patent: WO 99/01545 (1999-01-01), None
patent: WO 99/35274 (1999-07-01), None
patent: WO 99/49022 (1999-09-01), None
Broun et al., Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science, 1998, vol. 282: 1315-1317.
Devos et al., Practical limits of function prediction: Proteins: Structure, function, and Genetics, 2000, vol. 41: 98-107.
Guo et al., Protein tolerance to random amino acid change. PNAS., 2004, vol. 101 (25): 9205-9210.
Seffernick et al., Melamine deaminase and Atrazine chlorohydrolase: 98 percent identical but functionally different. J. Bacteriol.,2001, vol. 183 (8): 2405-2410.
Whisstock et al., Prediction of protein function from protein sequence. Q. Rev. Biophysics., 2003, vol. 36 (3): 307-340.
Witkowski et al., Conversion of b-ketoacyl synthase to a Malonyl Decarboxylase by replacement of the active cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650.
Yamashita et al., J. Biochem., vol. 116, pp. 1233-1240 (1994).
Chang et al., J. Mol. Biol., vol. 288, pp. 623-634 (1999).
Abstract of article by Fierobe et al., Biochemistry, vo. 35, No. 26, pp. 8696-8704 (1996).
Abstract of Article by Nakamura et al., Protein Eng., vol. 10, No. 11, pp. 1263-1269 (1997).
Wantabe et al., Journal of Molecular Catalysis B: Enzymatic, vol. 4, pp. 167-180 (1998).
Wantanabe et al., Applied and Environmental Microbiology, vol. 62, pp. 2066-2073.
Katsuragi et al., Gen Bank Acc. No. A26879 (1988).
Kosei et al., Abstract, Enzyme and Microbiol. Technology, vol. 19, No. 6, pp. 456-461 (1996).
Chang et al., Database CAPLUS, Accession No. 1999-297845 (1999).
Fierobe et al., Biochemistry, vol. 35, No. 26, pp. 8696-8704 (1996).
Fierobe et al., Database CAPLUS, Accession No. 1996-345793 (1996).
Katsuragi et al., Database GenBank, Accession No. A26879 (1988).
Nakamura et al., Protein Engineering, vol. 10, No. 11, pp. 1263-1269 (1997).
Nakamura et al., Database CAPLUS—Accession No. 1998-168593 (1998).
Takahashi et al. Enzyme and Microbial Technology, vol. 19, pp. 456-461 (1996).
Takahashi et al., Database Biosis, Accession No. B-1996-699229701 (1996).
Watanabe et al., Database CAPLUS, Accession No. 1996-335321 (1996).
Watanabe et al., Database CAPLUS, Accession No. 1998-380755 (1998).

Affiliated with

Also associated with

Rating

Starch debranching enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Starch debranching enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Starch debranching enzymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4203095