Starch branching enzyme

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S284000, C800S295000, C536S023200, C536S023600

Reexamination Certificate

active

07667114

ABSTRACT:
This invention relates to a new starch branching enzyme, and to the gene encoding the enzyme. In particular, the invention provides a new starch branching enzyme type II from wheat, the nucleic acid encoding the enzyme, and constructs comprising the nucleic acid. The invention also relates to a novel method for identification of branching enzyme type II proteins, which is useful for screening wheat germplasm for null or altered alleles of wheat branching enzyme IIb. The novel gene, protein and methods of the invention are useful in production of plants which produce grain with novel properties for food and industrial applications, for example wheat grain containing high amylose or low amylopectin starch.

REFERENCES:
patent: 5866793 (1999-02-01), Båga et al.
patent: 6730825 (2004-05-01), Goldsbrough et al.
patent: 6916976 (2005-07-01), Li et al.
patent: WO 97/22703 (1997-06-01), None
patent: WO 00/15810 (2000-03-01), None
Baga et al 1999 Starch 51:11-116, provided by Applicant in IDS.
Supplemental European Search Report of EP 01 90 7236.2.
Matoko Takaoka et al., “Structural Characterization of High Molecular Weight Starch Granule-Bound Proteins in Wheat (Triticum aestivum L.)”, J. Agric. Food Chem., 1997, 45, pp. 2929-2934.
Matthew K. Morrell et al., “Differential Expression and Properties of Starch Branching Enzyme Isoforms in Developing Wheat Endosperm1”, Plant Physiol. (1997), 113: 201-208.
Sadequr Rahman et al. “The Major Proteins of Wheat Endosperm Starch Granules”, Aust. J. Plant Physiol., 1995, 22: 793-803.
Sadequr Rahman et al., “Comparison of Starch-Branching Enzyme Genes Reveals Evolutionary Relationships Among Isoforms. Characterization of a Gene for Starch-Branching Enzyme IIa from the Wheat D Gemome DonorAegilops tauschii1”, Plant Physiology, Mar. 2001, vol. 125, pp. 1314-1324.
Monica Båga et al., “Wheat Starch Modification Through Biotechnology”, Starch/Stärke 51 (1999) Nr. 4 S. 111-116.

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